Isoprenoid biosynthesis in dandelion latex is enhanced by the overexpression of three key enzymes involved in the mevalonate pathway

BMC Plant Biology
Katharina M PütterChristian Schulze Gronover

Abstract

Latex from the dandelion species Taraxacum brevicorniculatum contains many high-value isoprenoid end products, e.g. triterpenes and polyisoprenes such as natural rubber. The isopentenyl pyrophosphate units required as precursors for these isoprenoids are provided by the mevalonate (MVA) pathway. The key enzyme in this pathway is 3-hydroxy-methyl-glutaryl-CoA reductase (HMGR) and its activity has been thoroughly characterized in many plant species including dandelion. However, two enzymes acting upstream of HMGR have not been characterized in dandelion latex: ATP citrate lyase (ACL), which provides the acetyl-CoA utilized in the MVA pathway, and acetoacetyl-CoA thiolase (AACT), which catalyzes the first step in the pathway to produce acetoacetyl-CoA. Here we isolated ACL and AACT genes from T. brevicorniculatum latex and characterized their expression profiles. We also overexpressed the well-characterized HMGR, ACL and AACT genes from Arabidopsis thaliana in T. brevicorniculatum to determine their impact on isoprenoid end products in the latex. The spatial and temporal expression profiles of T. brevicorniculatum ACL and AACT revealed their pivotal role in the synthesis of precursors necessary for isoprenoid biosynthesis in latex...Continue Reading

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Citations

Jul 7, 2018·Plant Biotechnology Journal·Chase KempinskiJoe Chappell
Jul 26, 2019·The Plant Journal : for Cell and Molecular Biology·Eva NiephausChristian Schulze Gronover
Apr 2, 2020·Annual Review of Biochemistry·Satoshi Yamashita, Seiji Takahashi
May 30, 2019·International Journal of Molecular Sciences·Quanliang XieXuchu Wang
Jun 28, 2019·Plant Direct·Kristina UnlandChristian Schulze Gronover
Nov 20, 2019·Journal of Experimental Botany·Vincent Alexander BenninghausChristian Schulze Gronover
Jan 15, 2019·Critical Reviews in Food Science and Nutrition·K Srinivasan, Elna M Buys
Apr 17, 2020·Journal of Experimental Botany·Iris LangeDuroy A Navarre
Jul 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nicole van DeenenChristian Schulze Gronover
Apr 13, 2019·Scientific Reports·Katharina M PütterChristian Schulze Gronover
Aug 31, 2021·Current Opinion in Plant Biology·Arielle R JohnsonMargaret H Frank

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Datasets Mentioned

BETA
GO664824
DR401254
DR402930
KY765686
KY765687
DR398498
KY765685
DR398619
PS01217
PS01216

Methods Mentioned

BETA
transgenic
nuclear magnetic resonance

Software Mentioned

OriginPro
Clustal Omega
Rad CFX Manager
Leica Application Suite X

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