Isoprenylation of the G protein gamma subunit is both necessary and sufficient for beta gamma dimer-mediated stimulation of phospholipase C

Biochemistry
A DietrichP Gierschik

Abstract

We have previously shown that isoprenylation and/or additional post-translational processing of the G protein gamma 1 subunit carboxyl terminus is required for beta 1 gamma 1 subunit stimulation of phospholipase C-beta 2 (PLC beta 2) [Dietrich, A., Meister, M., Brazil, D., Camps, M., & Gierschik, P. (1994) Eur. J. Biochem. 219, 171-178]. To examine whether isoprenylation of the gamma 1 subunit alone is sufficient for beta 1 gamma 1-mediated PLC beta 2 stimulation or whether any of the two subsequent modifications, proteolytic removal of the carboxyl-terminal tripeptide and/or carboxylmethylation, is required for this effect, nonisoprenylated recombinant beta 1 gamma 1 dimers were produced in baculovirus-infected insect cells, purified to near homogeneity, and then isoprenylated in vitro using purified recombinant protein farnesyltransferase. Analysis of the beta 1 gamma 1 dimer after in vitro farnesylation by reversed phase high-performance liquid chromatography followed by delayed extraction matrix-assisted laser desorption/ionization mass spectrometry confirmed that the gamma 1 subunit was carboxyl-terminally farnesylated but not proteolyzed and carboxylmethylated. Functional reconstitution of in vitro-farnesylated beta 1 gam...Continue Reading

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Citations

Apr 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Maria H HamiltonJohn D Hildebrandt
Jun 29, 1999·European Journal of Cell Biology·I BrunkG Ahnert-Hilger
Nov 18, 2005·Journal of Biochemical and Molecular Toxicology·Nazarius S Lamango
Sep 30, 2009·Cellular Signalling·Timothy MulliganSteven A Farber
Jun 3, 2004·The Journal of Biological Chemistry·Zhixian ZhangTheodore G Wensel
Feb 14, 2006·The Journal of Biological Chemistry·Zairong LiRobert F Margolskee
Jul 25, 2013·Molecular Pharmacology·Angeline M Lyon, John J G Tesmer
May 1, 2019·Annual Review of Plant Biology·Sona Pandey
Mar 27, 2002·The Journal of Biological Chemistry·Muslum AkgozN Gautam
Jan 11, 2021·Chemistry and Physics of Lipids·Kaushik MuralidharanAngeline M Lyon
Mar 17, 1999·Analytical Biochemistry·J L KerwinJ R Yates
Jan 1, 1997·Annual Review of Pharmacology and Toxicology·D E Clapham, E J Neer

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