Isoprostanes constrict human radial artery by stimulation of thromboxane receptors, Ca2+ release, and RhoA activation

The Journal of Thoracic and Cardiovascular Surgery
Irem MueedLuke J Janssen

Abstract

Radial artery vasospasm remains a potential cause of early graft failure after coronary bypass graft surgery, despite pretreatment with alpha-adrenergic or calcium channel blockers. We examined the roles of isoprostanes and prostanoid receptors selective for thromboxane A2 in the vasoconstriction of human radial arteries. Human radial arterial segments were pretreated intraoperatively with verapamil/papaverine or nitroglycerine/phenoxybenzamine, or not treated. In the laboratory, we measured isometric contractions in ring segments, vasoconstriction in pressurized segments, and changes in [Ca2+] and K+ currents in single cells. Although phenoxybenzamine eliminated adrenergic responses, the isoprostane 15-F(2t)-IsoP and 2 closely related E-ring molecules (15-E(1t)-IsoP and 15-E(2t)-IsoP) still evoked powerful contractions; 15-E(2t)-IsoP was approximately 10-fold more potent than the other 2 agents. Responses were mediated through thromboxane receptors because they were sensitive to ICI-192605. Furthermore, they were sensitive to the Rho-kinase inhibitors Y-27632 or H-1152 (both 10(-5) mol/L) or to cyclopiazonic acid (which depletes the internal Ca2+ pool), but not to nifedipine. In single cells, 15-E(2t)-IsoP elevated [Ca2+]i and...Continue Reading

References

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May 24, 2006·Cardiovascular Research·Yongde ZhangLuke J Janssen

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Citations

Apr 24, 2012·Interactive Cardiovascular and Thoracic Surgery·Mustafa DoganOsman Gokalp
Nov 22, 2015·The Annals of Thoracic Surgery·Guo-Wei He, David P Taggart
May 7, 2013·Prostaglandins & Other Lipid Mediators·Jean-Marie GalanoThierry Durand
May 14, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Saskia van der Sterren, Eduardo Villamor
Feb 15, 2011·Translational Research : the Journal of Laboratory and Clinical Medicine·Irem MueedLuke J Janssen
Sep 23, 2008·Free Radical Biology & Medicine·Ghazaleh Esmaeil PourmahramJeremy P T Ward
Sep 22, 2009·Canadian Journal of Physiology and Pharmacology·Olcay Murat DisliAhmet Parlak
Oct 25, 2008·Circulation Research·Heinrich Sauer, Maria Wartenberg

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