Isoproterenol stimulates 5'-AMP-activated protein kinase and fatty acid oxidation in neonatal hearts

American Journal of Physiology. Heart and Circulatory Physiology
Jagdip S JaswalGary D Lopaschuk

Abstract

Isoproterenol increases phosphorylation of LKB, 5'-AMP-activated protein kinase (AMPK), and acetyl-CoA carboxylase (ACC), enzymes involved in regulating fatty acid oxidation. However, inotropic stimulation selectively increases glucose oxidation in adult hearts. In the neonatal heart, fatty acid oxidation becomes a major energy source, while glucose oxidation remains low. This study tested the hypothesis that increased energy demand imposed by isoproterenol originates from fatty acid oxidation, secondary to increased LKB, AMPK, and ACC phosphorylation. Isolated working hearts from 7-day-old rabbits were perfused with Krebs solution (0.4 mM palmitate, 11 mM glucose, 0.5 mM lactate, and 100 mU/l insulin) with or without isoproterenol (300 nM). Isoproterenol increased myocardial O(2) consumption (in J·g dry wt(-1)·min(-1); 11.0 ± 1.4, n = 8 vs. 7.5 ± 0.8, n = 6, P < 0.05), and the phosphorylation of LKB (in arbitrary density units; 0.87 ± 0.09, n = 6 vs. 0.59 ± 0.08, n = 6, P < 0.05), AMPK (0.82 ± 0.08, n = 6 vs. 0.51 ± 0.06, n = 6, P < 0.05), and ACC-β (1.47 ± 0.14, n = 6 vs. 0.97 ± 0.07, n = 6, P < 0.05), with a concomitant decrease in malonyl-CoA levels (in nmol/g dry wt; 0.9 ± 0.9, n = 8 vs. 7.5 ± 1.3, n = 8, P < 0.05) and inc...Continue Reading

References

Jun 1, 1978·Circulation Research·H S Klopfenstein, A M Rudolph
Dec 1, 1992·Cardiovascular Research·G D LopaschukT Itoi
Dec 1, 1991·The American Journal of Physiology·G D LopaschukD R Marsh
Nov 1, 1991·Analytical Biochemistry·D Constantin-TeodosiuE Hultman
Sep 1, 1985·Pediatric Research·D F TeitelA M Rudolph
Nov 1, 1994·Clinical Pharmacokinetics·C Steinberg, D A Notterman
Nov 1, 1994·The American Journal of Physiology·R L Collins-NakaiG D Lopaschuk
Apr 1, 1996·The American Journal of Physiology·R Tian, J S Ingwall
Oct 29, 1998·The Journal of Biological Chemistry·G W GoodwinH Taegtmeyer
Oct 12, 1999·The American Journal of Physiology·G W Goodwin, H Taegtmeyer
Oct 28, 1999·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·M J TurnerA A Ehsani
Nov 24, 1999·Molecular and Cellular Biochemistry·D W SchwertzC Ryjewski
May 19, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·F J Sutherland, D J Hearse
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·A R PanchalW C Stanley
Sep 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·A R PanchalW C Stanley
Oct 15, 1971·FEBS Letters·Colleen M. Smith, John R. Williamson
Oct 15, 1971·FEBS Letters·Francis E. SluseJoseph M. Tager
May 7, 2002·Exercise and Sport Sciences Reviews·Lawrence L Spriet, George J F Heigenhauser
Sep 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Myriame PoirierChristine Des Rosiers
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Arzu Onay-BesikciArzu Onay Besikci
Nov 21, 2002·Biochemical Society Transactions·M R Munday
Apr 5, 2003·American Journal of Physiology. Endocrinology and Metabolism·Mary C Sugden, Mark J Holness
Jul 16, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Gen TakagiDorothy E Vatner
Dec 4, 2003·Biochemical Society Transactions·M J Holness, M C Sugden
Dec 4, 2003·Biochemical Society Transactions·D CarlingH Whitrow
Jun 8, 2004·The Journal of Biological Chemistry·Aneta E ReszkoHenri Brunengraber
Oct 28, 2004·The Journal of Biological Chemistry·Judith Y AltarejosGary D Lopaschuk

❮ Previous
Next ❯

Citations

Apr 27, 2013·Apoptosis : an International Journal on Programmed Cell Death·Xiao-Zhen ZhuoPing Song
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Juliano L SartorettoThomas Michel
Jan 17, 2016·Journal of Molecular and Cellular Cardiology·Evangelos P DaskalopoulosSandrine Horman
Mar 19, 2011·Biochemical and Biophysical Research Communications·Catherine L PassarielloClaudio Stefanelli
Jan 11, 2012·Biochimica Et Biophysica Acta·Catherine L PassarielloClaudio Stefanelli
Feb 12, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Jun MoriGavin Y Oudit
May 21, 2015·Journal of Cell Science·Xiaojing HouQiang Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.