PMID: 2105498Feb 1, 1990Paper

Isoproterenol stimulates shift of G proteins from plasma membrane to pinocytotic vesicles in rat adipocytes: a possible means of signal dissemination

Proceedings of the National Academy of Sciences of the United States of America
K Haraguchi, M Rodbell

Abstract

Guanine nucleotide-binding regulatory proteins (G proteins) are linked to a large number of surface membrane receptors and appear to regulate a variety of effector systems located both in the plasma membrane and in other parts of the cell. The mechanism of the disseminative actions of G proteins remains obscure. During an investigation of the fate of two types of G proteins, Gs and Gi, in rat adipocytes, we unexpectedly found that isoproterenol, which stimulates cAMP levels and lipolysis in these cells, induces parallel increases in both Gs and Gi in a low-density microsomal fraction rich in endosomes and Golgi bodies. Two plasma membrane constitutive enzymes, adenylyl cyclase and 5'-nucleotidase, are also elevated in this fraction. NaF and NaN3, metabolic inhibitors, block the redistribution process. The isoproterenol-stimulated shifts are completely reversible after removal of the hormone, indicating a recycling, endocytic process. The endocytic process seems to be fluid phase endocytosis, or pinocytosis, since isoproterenol stimulates the uptake of both fluorescent-labeled dextran and horseradish peroxidase into the same vesicles containing Gs. However, the vesicles that accumulate in response to isoproterenol seem heterogen...Continue Reading

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Citations

Aug 1, 1993·Journal of Cellular Biochemistry·N McFarlane-AndersonN Bégin-Heick
Jan 1, 1995·Journal of Cancer Research and Clinical Oncology·V N GorelovP E Goretzki
Sep 11, 1991·Trends in Biochemical Sciences·A M SpiegelW F Simonds
Jan 1, 1996·Trends in Endocrinology and Metabolism : TEM·A P BevanB I Posner
Mar 1, 1994·Acta histochemica·W SchulzeL Will-Shahab
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K Haraguchi, M Rodbell
Aug 1, 1994·Journal of Neuroendocrinology·J C PainsonR Collu
Oct 23, 1998·Pharmacology & Toxicology·S P SinghR Ravindra
Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·P HuangM J Stutts
Apr 24, 1995·FEBS Letters·E S BursteinM R Brann
Sep 1, 2005·The FEBS Journal·Clémence MerlenFrançois Authier

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