Isorhapontigenin ameliorates cerebral ischemia/reperfusion injury via modulating Kinase Cε/Nrf2/HO-1 signaling pathway.

Brain and Behavior
Zhe XueBainan Xu

Abstract

Isorhapontigenin (ISO) has been shown to have antioxidant activity. This study aimed to investigate the antioxidant effects of ISO on cerebral ischemia/reperfusion (I/R) injury and its possible molecular mechanisms. Focal cerebral ischemia-reperfusion injury (MCAO/R) model and primary cortical neurons were established an oxygen-glucose deprivation (OGD / R) injury model. After 24 hr of reperfusion, the neurological deficits of the rats were analyzed and HE staining was performed, and the infarct volume was calculated by TTC staining. In addition, the reactive oxygen species (ROS) in rat brain tissue, the content of 4-Hydroxynonenal (4-HNE), and 8-hydroxy2deoxyguanosine (8-OHdG) were detected. Neuronal cell viability was determined by MTT assay. Western blot analysis was determined for protein expression. ISO treatment significantly improved neurological scores, reduced infarct volume, necrotic neurons, ROS production, 4-HNE, and 8-OHdG levels. At the same time, ISO significantly increased the expression of Nrf2 and HO-1. The neuroprotective effects of ISO can be eliminated by knocking down Nrf2 and HO-1. In addition, knockdown of the PKCε blocked ISO-induced nuclear Nfr2, HO-1 expression. ISO protected against oxidative damage ...Continue Reading

References

Feb 24, 2000·The Journal of Pathology·D L Carden, D N Granger
May 13, 2015·Journal of the Neurological Sciences·Prabodh SadanaAlexander Mdzinarishvili
Nov 26, 2015·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Li ZhengWeiguang Zhang
Jan 20, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chao ZhuZhihua Cui
Mar 3, 2017·Journal of Natural Science, Biology, and Medicine·Itishri JenaSubrat Kumar Sahoo
May 21, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xu ZhangJunguo Duan
Dec 8, 2017·Scientific Reports·Karina Luiza Dias-TeixeiraUlisses G Lopes
Feb 2, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Vishal R YadavYong-Xiao Wang
Feb 13, 2018·Human Molecular Genetics·Ravi ChopraVikram G Shakkottai
May 22, 2018·Journal of Ethnopharmacology·Jiangmei WangYonghua Wang

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