Isotachophoresis at pH extremes: theory and experimental validation

Electrophoresis
S V ErmakovP G Righetti

Abstract

The evolution of an isotachophoresis (ITP) system in acidic or basic pH ranges can be quite different from that predicted by the existing theory. It was found theoretically and proved experimentally that the contribution of hydrogen or hydroxyl ion to conductivity of solution and/or its net charge changes the behavior of the ITP system, creating in the terminating electrolyte an additional zone close to the initial interfaces between electrolytes (leader and terminator). One boundary of the zone, being either sharp or dispersed, moves toward the leader; the other is always sharp and stationary and coincides with initial electrolytes' discontinuity. The latter can be registered in the presence of electroosmotic flow which delivers it to the detection point. In order to describe the dynamics of the ITP system at pH extremes an algorithm of analytical solution was developed, based on the revised Kohlrausch theory. Its predictions coincide well with computer simulations and experimental data. The results presented can help in a correct analysis of ITP data and explain some confusing phenomena which were considered to be artifacts.

References

Apr 1, 1980·British Journal of Urology·A LindnerM Many
Jun 1, 1996·Electrophoresis·S V ErmakovP G Righetti

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Citations

Feb 24, 2001·Electrophoresis·P Gebauer, P Bocek
Dec 15, 2004·Electrophoresis·Bohuslav Gas, Ernst Kenndler
Jun 12, 2009·Electrophoresis·Bohuslav Gas
Jun 12, 2009·Electrophoresis·Wolfgang ThormannRichard A Mosher
Mar 9, 2006·Electrophoresis·Vlastimil HruskaBohuslav Gas
Jan 22, 2009·Electrophoresis·Vlastimil HruskaGyula Vigh
Dec 21, 2006·Electrophoresis·Vlastimil Hruska, Bohuslav Gas
Apr 25, 2012·Electrophoresis·Supreet S Bahga, Juan G Santiago
Mar 2, 2010·Electrophoresis·Wolfgang ThormannRichard A Mosher

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