PMID: 19916946Nov 18, 2009Paper

Isotachophoresis of nucleic acids in agarose gel rods

Biochemistry. Biokhimii︠a︡
Valentina N KondratovaAnatoly V Lichtenstein

Abstract

A new method of electrophoresis (isotachophoresis in agarose gel rods) in which nucleic acid molecules are not separated but, oppositely, are brought together into one band, was elaborated. Heterogeneous in size DNA and RNA polymers present in a few milliliters of a solution at so low concentration that their isolation by other methods is hardly attainable and fraught with losses are brought together into one visible narrow band when put in a discontinuous electric field. Polynucleotides migrate in dilute (0.1%) semifluid agarose gel that permits easy quantitative isolation of the band of interest. Resulting DNA can be used directly in PCR. The suggested method for isolation of micro amounts of nucleic acids from dilute solutions can be applied to forensic and clinical research and cancer gene diagnostics by the analysis of fragmented circulating DNA from bodily fluids.

References

Sep 1, 1996·Nature Medicine·H NawrozD Sidransky
Oct 3, 1999·Cancer Metastasis Reviews·P AnkerM Stroun
May 7, 2002·Nature Reviews. Cancer·David Sidransky
Sep 26, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Gabriella SozziUgo Pastorino
Nov 25, 2003·Expert Review of Molecular Diagnostics·Blenda C K Wong, Y M Dennis Lo
Nov 13, 2004·Clinical Chemistry·Luis J HerreraTony E Godfrey
Jul 26, 2005·Clinical Chemistry·Bernd SchmidtMichael Fleischhacker
Nov 30, 2005·BioTechniques·Valentina N KondratovaAnatoly V Lichtenstein
Dec 20, 2007·Clinical Chemistry·Catherine Lofton-DayAndrew Sledziewski
May 8, 2008·Journal of Cancer Research and Clinical Oncology·Vanesa GarciaFelix Bonilla
Jun 7, 2008·BioTechniques·Hao Ma, Stephen Difazio
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Aug 2, 2008·Nature Medicine·Frank DiehlLuis A Diaz
Oct 8, 2008·Annals of the New York Academy of Sciences·Hovsep S MelkonyanSamuil R Umansky
Nov 13, 2008·PloS One·Melissa Piper HunterClay B Marsh
Feb 3, 2009·Clinical Chemistry·Julia BeckEkkehard Schütz
Feb 3, 2009·Clinical Chemistry·Eugene M ShekhtmanSamuil R Umansky

❮ Previous
Next ❯

Citations

Jan 1, 1975·Biulleten' eksperimental'noĭ biologii i meditsiny·M A ShliankevichV S Shapot
Apr 1, 1975·Biulleten' eksperimental'noĭ biologii i meditsiny·V A Beniush, L P Tupitsyna
Jan 22, 2014·Journal of Chromatography. a·Anita RogacsJuan G Santiago
Sep 11, 2010·Analytical Biochemistry·Valentina N KondratovaAnatoly V Lichtenstein
Jan 6, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Benjamin P SullivanJonathan D Posner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.