Isotope dilution gas chromatography/mass spectrometry method for the determination of methionine sulfoxide in protein

Analytical Chemistry
M A SochaskiJ W Baynes

Abstract

We have developed a new technique for quantifying methionine sulfoxide (MetSO) in protein to assess levels of oxidative stress in physiological systems. In this procedure, samples are hydrolyzed with methanesulfonic acid (MSA) in order to avoid the conversion of MetSO to methionine (Met) that occurs during hydrolysis of protein in HCl. The hydrolysate is fractionated on a cation exchange column to remove the nonvolatile MSA from amino acids, and the amino acids are then derivatized as their trimethylsilyl esters for analysis by selected ion monitoring-gas chromatography/mass spectrometry. The limit of detection of the assay is 200 pmol of MetSO per analysis, and the interassay coefficient of variation is 5.8%. Compared to current methods, the SIM-GC/MS assay avoids the potential for conversion of Met to MetSO during sample preparation, requires less sample preparation time, has lower variability, and uses mass spectrometry for sensitive and specific analyte detection.

Citations

Sep 25, 2004·Mass Spectrometry Reviews·Isabella Dalle-DonneAldo Milzani
May 3, 2005·Experimental Eye Research·R J Truscott
Oct 31, 2012·The British Journal of Nutrition·S M Rutherfurd, P J Moughan
Oct 27, 2009·The Journal of Biological Chemistry·Xingjun FanVincent M Monnier
May 18, 2010·Progress in Retinal and Eye Research·R J Truscott, Xiangjia Zhu
Sep 2, 2008·Biochimica Et Biophysica Acta·Peter G Hains, R J Truscott
Sep 1, 2015·Biochimica Et Biophysica Acta·R J Truscott, Michael G Friedrich
Jan 25, 2014·Proteomics. Clinical Applications·R J Truscott, Michael G Friedrich
Jul 19, 2016·Trends in Biochemical Sciences·R J TruscottMichael G Friedrich
Jul 23, 2005·Annals of the New York Academy of Sciences·J W C BrockJ W Baynes
Jan 29, 2002·Journal of Mass Spectrometry : JMS
Dec 22, 2005·Mass Spectrometry Reviews·Philippe A Guy, François Fenaille
Nov 26, 2009·Current Protocols in Protein Science·S M Rutherfurd, G Sarwar Gilani

Related Concepts

Methionine sulfoxide,35S-labeled, (+-)-isomer
N(6)-carboxymethyllysine
Zyderm
Eye Lens Protein
Cyanogen Bromide
LDL-1
Enisyl
Chromatography, Gas-Liquid-Mass Spectrometry
Pedameth
Gene Products, Protein

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.