Isotope dilution gas chromatography/mass spectrometry method for the determination of methionine sulfoxide in protein

Analytical Chemistry
M A SochaskiJ W Baynes

Abstract

We have developed a new technique for quantifying methionine sulfoxide (MetSO) in protein to assess levels of oxidative stress in physiological systems. In this procedure, samples are hydrolyzed with methanesulfonic acid (MSA) in order to avoid the conversion of MetSO to methionine (Met) that occurs during hydrolysis of protein in HCl. The hydrolysate is fractionated on a cation exchange column to remove the nonvolatile MSA from amino acids, and the amino acids are then derivatized as their trimethylsilyl esters for analysis by selected ion monitoring-gas chromatography/mass spectrometry. The limit of detection of the assay is 200 pmol of MetSO per analysis, and the interassay coefficient of variation is 5.8%. Compared to current methods, the SIM-GC/MS assay avoids the potential for conversion of Met to MetSO during sample preparation, requires less sample preparation time, has lower variability, and uses mass spectrometry for sensitive and specific analyte detection.

References

Jan 1, 1977·Methods in Enzymology·W E Savige, A Fontana
Oct 7, 1975·Biochemistry·Y ShechterA Patchornik
Oct 1, 1992·Free Radical Biology & Medicine·H EsterbauerG Jürgens
Jun 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·E L Bierman
Oct 16, 1980·Biochemical and Biophysical Research Communications·P S Wong, J Travis
May 1, 1983·Archives of Biochemistry and Biophysics·N Brot, H Weissbach
Feb 1, 1983·Mechanisms of Ageing and Development·H J HorstmannK Sames
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M H Garner, A Spector
Jan 1, 1995·Methods in Enzymology·K L MaierU Costabel
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B N AmesT M Hagen
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·R L LevineE R Stadtman
Dec 1, 1996·Experimental Eye Research·A L LundD L Smith
May 1, 1997·Chemical Research in Toxicology·E R Stadtman, B S Berlett
Jun 9, 1999·Mechanisms of Ageing and Development·R L LevineE R Stadtman

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Citations

Sep 25, 2004·Mass Spectrometry Reviews·Isabella Dalle-DonneAldo Milzani
May 3, 2005·Experimental Eye Research·Roger J W Truscott
Oct 31, 2012·The British Journal of Nutrition·Shane M Rutherfurd, Paul J Moughan
Oct 27, 2009·The Journal of Biological Chemistry·Xingjun FanVincent M Monnier
May 18, 2010·Progress in Retinal and Eye Research·Roger J W Truscott, Xiangjia Zhu
Sep 2, 2008·Biochimica Et Biophysica Acta·Peter G Hains, Roger J W Truscott
Sep 1, 2015·Biochimica Et Biophysica Acta·Roger J W Truscott, Michael G Friedrich
Jan 25, 2014·Proteomics. Clinical Applications·Roger J W Truscott, Michael G Friedrich
Jul 19, 2016·Trends in Biochemical Sciences·Roger J W TruscottMichael G Friedrich
Jul 23, 2005·Annals of the New York Academy of Sciences·J W C BrockJ W Baynes
Jan 29, 2002·Journal of Mass Spectrometry : JMS
Dec 22, 2005·Mass Spectrometry Reviews·Philippe A Guy, François Fenaille
Nov 26, 2009·Current Protocols in Protein Science·Shane M Rutherfurd, G Sarwar Gilani
Sep 11, 2007·Journal of Proteome Research·Peter G Hains, Roger J W Truscott
Mar 31, 2005·Journal of Agricultural and Food Chemistry·Marie A SegurelRaymond L Baumes
Jan 29, 2005·Journal of Agricultural and Food Chemistry·Wesley G TaylorPaul G Fields

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