PMID: 7011363Feb 3, 1981Paper

Isotope-exchange evidence for an ordered mechanism for rat-liver glucokinase, a monomeric cooperative enzyme

Biochemistry
M GregoriouA Cornish-Bowden

Abstract

The order of addition of substrates and release of products in the reaction catalyzed by rat-liver glucokinase has been studied by measurements of isotope exchange. Experiments at chemical equilibrium showed some degree of randomness, but steady-state experiments showed a predominantly ordered process with glucose binding first and glucose 6-phosphate released last. Experiments to trap binary complexes in the steady state demonstrated the existence of complexes of the enzyme with glucose and with glucose 6-phosphate but gave no evidence for the occurrence of corresponding complexes with ATP or ADP. Flux ratios measured in both the forward and reverse reactions provided a more rigorous and quantitative confirmation of these characteristics of the reaction. These observations support the interpretation of glucokinase cooperativity in terms of a "mnemonical" mechanism and conflict with an alternative interpretation in terms of a random addition of substrates.

References

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Citations

Oct 1, 1995·Journal of Bioenergetics and Biomembranes·J H Hofmeyr
Dec 12, 2001·European Journal of Biochemistry·A Cornish-Bowden, M L Cárdenas
Aug 1, 1983·European Journal of Biochemistry·M GregoriouA Cornish-Bowden
May 15, 1984·European Journal of Biochemistry·D Pollard-Knight, A Cornish-Bowden
Jan 2, 1986·European Journal of Biochemistry·G Pettersson
Nov 17, 1986·European Journal of Biochemistry·M GregoriouA Cornish-Bowden
Jul 15, 1987·European Journal of Biochemistry·J Ricard, A Cornish-Bowden
Nov 26, 1990·European Journal of Biochemistry·G Pettersson
Nov 24, 2011·Archives of Biochemistry and Biophysics·Mioara Larion, Brian G Miller
Mar 1, 1985·Archives of Biochemistry and Biophysics·D Pollard-KnightI P Trayer
Apr 16, 1998·Biochimica Et Biophysica Acta·M L CárdenasT Ureta
Jan 23, 1990·Biochemistry·K E NeetP S Tippett
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·T W Traut
Jan 1, 1983·European Journal of Biochemistry·M K Darby, I P Trayer
Jan 7, 1987·Journal of Theoretical Biology·A Cornish-Bowden, M L Cárdenas

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