Isotope ratio mass spectrometry, compared with conventional mass spectrometry in kinetic studies at low and high enrichment levels: application to lipoprotein kinetics

Analytical Biochemistry
F PontB Vergès

Abstract

The aim of the present study was to compare the performances of gas chromatography/mass spectrometry (GC/MS) and gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) in stable isotope kinetic studies. In the analysis of cholesterol and leucine, GC/C/IRMS gave precise and linear results over a large scale of 13C enrichment (-22 to +760 delta/1000 for cholesterol, -26 to +600 delta/1000 for leucine). Compared with GC/MS, GC/C/IRMS was much more accurate and reproducible, especially at low [13C]-cholesterol enrichment (-12 delta/1000), with cholesterol samples ranging from 0.11 to 17 ng. Cholesterol ester kinetics in rabbit plasma low-density lipoproteins was studied after injection of 3 mg [3,4-(13)C]cholesterol. A smooth and regular kinetic curve was obtained with GC/C/IRMS; results were much less reproducible with GC/MS. Finally, the performances of GC/C/IRMS were demonstrated in the simultaneous kinetic study of three human plasma apolipoproteins during a primed constant infusion of 0.7 mg.kg-1.h-1 L-[l-13C]leucine. Kinetic curves were obtained in very-low-density lipoproteins and low-density lipoproteins for apolipoprotein B100, and in high-density lipoproteins for apolipoproteins AI and AIL.

References

May 1, 1989·Biomedical & Environmental Mass Spectrometry·G N ThompsonD Halliday
Jun 1, 1996·Analytical Chemistry·F P AbramsonY Teffera
Mar 1, 1996·Journal of Mass Spectrometry : JMS·W A Brand
Apr 1, 1996·The American Journal of Physiology·G C Tchoumkeu-Nzouessa, G Rebel

❮ Previous
Next ❯

Citations

Jan 9, 2004·Isotopes in Environmental and Health Studies·E PouteauL B Fay
Jun 23, 2001·Protein Science : a Publication of the Protein Society·I ApostolA J Mathews
Oct 4, 2005·The Journal of Clinical Investigation·Christophe MarçaisPhilippe Moulin
Jan 27, 2009·Journal of Lipid Research·Bruno VergèsLaurence Duvillard
Aug 21, 2002·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·F PontB Vergès
Aug 6, 2004·Current Opinion in Clinical Nutrition and Metabolic Care·Hans M H van Eijk, Nicolaas E P Deutz
Apr 1, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Bruno VergèsPhilippe Gambert
Oct 16, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Frédéric PontBruno Vergès
Jun 20, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·F PontP Gambert
Jul 27, 2007·Mass Spectrometry Reviews·Martin KussmannLaurent B Fay
Mar 19, 2013·Analytical and Bioanalytical Chemistry·Felicia SeichterBoris Mizaikoff
Apr 4, 2019·Rapid Communications in Mass Spectrometry : RCM·Neringa MannerheimNina Buchmann
Mar 11, 2000·Current Opinion in Clinical Nutrition and Metabolic Care·W Meier-Augenstein
Apr 4, 2014·The Journal of Clinical Endocrinology and Metabolism·Bruno VergèsRémy Burcelin
Aug 18, 2020·The Journal of Clinical Endocrinology and Metabolism·Véronique Ferchaud-RoucherKhadija Ouguerram
Nov 17, 2011·Analytical and Bioanalytical Chemistry·Andreas WilkPeter Radermacher
Feb 12, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·H HasegawaT Hashimoto
Jun 23, 1999·Journal of Chromatography. a·W Meier-Augenstein

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.