PMID: 2494666Apr 1, 1989Paper

Isotype switching by a microinjected mu immunoglobulin heavy chain gene in transgenic mice

Proceedings of the National Academy of Sciences of the United States of America
J M DurdikErik Selsing

Abstract

Immunization of transgenic mice carrying an immunoglobulin mu heavy chain resulted in a response dominated by expression of the transgene variable region. Unexpectedly, in a large proportion of the antibody produced by immunized mice, the transgene variable region was associated with IgG rather than IgM. This demonstrates that the transgene can undergo an isotype switch. Four transgenic founder lines all exhibited transgene isotype switching despite the likelihood of random chromosomal integration of the transgene. In addition one of the lines was analyzed by breeding studies and the transgene was found to be genetically unlinked to the immunoglobulin heavy chain (Igh) locus. These results indicate that a precise chromosomal location is not required for isotype switching and suggest the possibility that the isotype switching process can occur interchromosomally.

References

Oct 1, 1979·The Journal of Experimental Medicine·Y Dohi, A Nisonoff
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J A LaskinP D Gottlieb
Aug 1, 1986·The Journal of Experimental Medicine·U StorbR L Brinster
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H M JäckM Wabl
Feb 1, 1986·Immunological Reviews·A RadbruchW Müller
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P F RobbinsA Nisonoff
Nov 20, 1987·Science·F W AltG D Yancopoulos
Aug 1, 1985·The Journal of Experimental Medicine·S A Tilley, B K Birshtein
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K L KnightW C Hanly
Dec 1, 1982·European Journal of Immunology·M SiekevitzA Marshak-Rothstein
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A P Perlmutter, W Gilbert
Jan 24, 1980·Nature·T H RabbittsD L Bentley
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T KataokaT Honjo
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R MakiS Tonegawa
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M ObataT Honjo

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Citations

Jan 1, 1995·Clinical Reviews in Allergy & Immunology·B A Chowdhury
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A ShimizuT Honjo
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A UmarP J Gearhart
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M ZhuM D Scharff
Jul 1, 1990·The Journal of Experimental Medicine·J HackettU Storb
Jul 11, 1992·The Journal of Experimental Medicine·J HackettU Storb
Feb 1, 1993·The Journal of Experimental Medicine·J SohnE Selsing
Nov 6, 2009·The Journal of Experimental Medicine·Wesley A DunnickF Nina Papavasiliou
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·N S GreenM D Scharff
Sep 1, 1991·The Journal of Allergy and Clinical Immunology·D Vercelli, R S Geha
Mar 15, 1991·Biochemical and Biophysical Research Communications·G FeriottoR Gambari
Jan 1, 1995·International Reviews of Immunology·N Lonberg, D Huszar
Aug 22, 1991·Nature·E Selsing
Jul 11, 1991·Nature
Jul 1, 1997·European Journal of Immunology·A GrandienA Coutinho
Jun 1, 1990·European Journal of Immunology·M J SharpeC Milstein
May 1, 1990·European Journal of Immunology·L Forni
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ning GaoDorothy Yuan
Dec 13, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicole D'AvirroErik Selsing
Dec 7, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wesley A DunnickJohn T Collins
Oct 21, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wesley A DunnickJohn T Collins
Jul 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A TerauchiT Azuma
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicole D'AvirroErik Selsing
Sep 29, 1995·Annals of the New York Academy of Sciences·F A Harding, N Lonberg
Oct 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katja AviszusLawrence J Wysocki

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