PMID: 8955580Nov 1, 1996Paper

Isotypic analysis of grass pollen-specific antibodies in human plasma. 4. Biological activity of allergen-specific and autoanti-IgE antibody fractions on basophil histamine release

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
T BatardB Basuyaux

Abstract

Blocking antibodies are defined as antibodies that compete with IgE for binding to allergens due to their specificity for those allergens. Thus, they may inhibit allergen-induced basophil and mast cell IgE-dependent mediator release both in vivo and in vitro. The present study was designed to evaluate the ability of antibodies isolated from human plasma samples on a Dactylis glomerata (Cocksfoot) pollen affinity-column to inhibit the Dactylis pollen-induced histamine release from human basophils (BHR) in vitro. Antibodies from Ig pools containing either high or low IgG4 anti-Dactylis pollen were purified on a Dactylis pollen affinity-column and then separated on an antihuman IgE column. Obtained Ig fractions were incubated for 30 min with Dactylis pollen allergens prior to incubation with basophils from Dactylis pollen-allergic donors. Cell supernatants were assessed for histamine content and the inhibition of BHR was calculated. Unlike control non-isolated Igs, the antibodies isolated on the Dactylis pollen column were able to inhibit efficiently and in a dose-dependent manner Dactylis pollen-induced BHR. The inhibitory activity was increased in isolated antibody samples that had high IgG4 levels. Antibodies isolated on the Da...Continue Reading

Citations

Apr 15, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K GehlharA Bufe
Dec 21, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·I Annila
Nov 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A M Denman

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