PMID: 3129416Dec 1, 1987

Isozymes of isocitrate dehydrogenase from an obligately psychrophilic bacterium, Vibrio sp. strain ABE-1: purification, and modulation of activities by growth conditions

Journal of Biochemistry
A IshiiH Shiokawa

Abstract

Each of the two isozymes, which are different in thermostability and quaternary structure, of isocitrate dehydrogenase (NADP+) [IDH: EC 1.1.1.42] was purified to an electrophoretically homogeneous state from an obligately psychrophilic marine bacterium, Vibrio sp. strain ABE-1. Hydrophobic chromatography was an efficient procedure to separate the two isozymes from each other. The isoelectric points of isozyme I (IDH-I; a dimer, Mr 88,100) and isozyme II (IDH-II; a monomer, Mr 80,500) were found to be pH 4.9 and 5.2, respectively. The two isozymes were similar in amino acid compositions, though there were slight differences in the contents of nonpolar and hydroxyl amino acids. However, their NH2-terminal amino acid sequences and immunochemical properties were clearly different from each other. The NH2-terminal amino acid sequence analysis also indicated that the subunits of IDH-I are chemically identical or highly homologous. Non-immuno-crossreactivity between the isozymes enabled us to measure the intracellular contents of the isozymes. IDH-I and -II were found to be differentially regulated in vivo by various growth conditions. IDH-I was induced by acetate, while IDH-II remained almost unchanged.

Citations

Jan 19, 1999·Annual Review of Microbiology·Alain J Cozzone
Oct 6, 2004·Preparative Biochemistry & Biotechnology·Oldrich JaniczekJitka Psotová
Nov 24, 2006·Extremophiles : Life Under Extreme Conditions·Corien BakermansMichael F Thomashow
Apr 28, 2017·Extremophiles : Life Under Extreme Conditions·Ryo HirotaYasuhiro Takada
Sep 27, 2008·Extremophiles : Life Under Extreme Conditions·Corien BakermansMichael F Thomashow
Jan 18, 2006·Journal of Molecular Microbiology and Biotechnology·Alain J Cozzone, Mansi El-Mansi
Jun 16, 2010·Archives of Microbiology·Shougo MatsuoYasuhiro Takada
Sep 21, 2011·Acta Crystallographica. Section D, Biological Crystallography·Navdeep S SidhuGeorge M Sheldrick
Aug 25, 2016·Nature Communications·Paul MurimaJohn D McKinney
Jan 19, 2006·Extremophiles : Life Under Extreme Conditions·Shinsuke MakiYasuhiro Takada

Related Concepts

Acetic Acids
Chromatography
SDS-PAGE
Immunodiffusion Measurement
Glyoxylates
Isocitrate Dehydrogenase-I
Isoelectric Point
Alloenzymes
Succinic Acids
Beneckea

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