Apr 9, 1981

Isozymes of ribonuclease in human serum and urine. I. Methodology and a survey of a control population

Clinica Chimica Acta; International Journal of Clinical Chemistry
J M Thomas, M E Hodes

Abstract

Methods are presented for the electrophoretic analysis of ribonuclease (RNase) enzymes in human serum and urine. Protocols for sample treatment, electrophoresis, and the RNase zymogram technique are described. With the application of these methods, RNase from serum and urine was separated into components differing on the basis of charge (charge isomers or "isozymes"), but not differing with respect to hydrolyzable sialic acid residues. Preliminary characterization of the electrophoretically separated components showed that some of the RNase species have different properties (pH optima and substrate preference). The major urine RNase isozymes appeared to be distinct from the major serum RNase isozymes. A survey of a control population indicated that the major serum and urine RNase enzymes are not genetically polymorphic.

  • References22
  • Citations5

References

  • References22
  • Citations5

Citations

Mentioned in this Paper

Urine
Enzymes, antithrombotic
SDS-PAGE
Sialic Acid Assay
Neuraminidase
Alkaline Ribonuclease
Enzymes for Treatment of Wounds and Ulcers
Pancreatic ribonuclease
Urine - SpecimenType
Enzymes, hematological

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