It is time for top-down venomics

The Journal of Venomous Animals and Toxins Including Tropical Diseases
Rafael D MelaniGilberto B Domont

Abstract

The protein composition of animal venoms is usually determined by peptide-centric proteomics approaches (bottom-up proteomics). However, this technique cannot, in most cases, distinguish among toxin proteoforms, herein called toxiforms, because of the protein inference problem. Top-down proteomics (TDP) analyzes intact proteins without digestion and provides high quality data to identify and characterize toxiforms. Denaturing top-down proteomics is the most disseminated subarea of TDP, which performs qualitative and quantitative analyzes of proteoforms up to ~30 kDa in high-throughput and automated fashion. On the other hand, native top-down proteomics provides access to information on large proteins (> 50 kDA) and protein interactions preserving non-covalent bonds and physiological complex stoichiometry. The use of native and denaturing top-down venomics introduced novel and useful techniques to toxinology, allowing an unprecedented characterization of venom proteins and protein complexes at the toxiform level. The collected data contribute to a deep understanding of venom natural history, open new possibilities to study the toxin evolution, and help in the development of better biotherapeutics.

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Aug 5, 2018·Rapid Communications in Mass Spectrometry : RCM·Parviz GhezellouBernhard Spengler
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Mar 7, 2021·Toxins·Stephanie Mouchbahani-Constance, Reza Sharif-Naeini

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Datasets Mentioned

BETA
PXD003403

Methods Mentioned

BETA
reversed-phase chromatography
size-exclusion chromatography
electrophoresis
hydrophobic interaction chromatography
ion exchange chromatography

Software Mentioned

TRASH
nTDP
ProSight PC
TopPIC
BUP
pTop
ProSight Lite
dTDP
Deconv
ProSight PTM

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