It takes acid, rather than ice, to freeze glucose

Scientific Reports
S A A van den BergK J M Boonen

Abstract

Plasma glucose levels provide the cornerstone of diabetes evaluation. Unfortunately, glucose levels drop in vitro due to glycolysis. Guidelines provide suitable conditions which minimize glycolysis, such as immediate centrifugation or the use of ice/water slurry storage containers. For obvious practical reasons, most laboratories use blood collection tubes containing glycolysis inhibitors. We describe the effect of a variety of commonly used blood collection tubes on in vitro stability of glucose. Furthermore, we looked at the validity of the assumption that glycolytic activity is minimal when blood is kept in an ice/water slurry. Sodium fluoride alone does not reduce in vitro glycolysis in the first 120 minutes after phlebotomy. Addition of citrate almost completely prevented in vitro glycolysis, but showed a positive bias (0.2 mmol/l) compared to control. This is partly due to a small drop in glucose level in control blood, drawn according to the current guidelines. This drop occurs within 15 minutes, in which glycolysis has been described to be minimal and acceptable. NaF-EDTA-citrate based test tubes provide the best pre-analytical condition available. Furthermore, glucose levels are not stable in heparinized blood placed i...Continue Reading

References

May 10, 2001·JAMA : the Journal of the American Medical Association·F de VegtR J Heine
Nov 9, 1961·The New England Journal of Medicine·J B FIELD, H E WILLIAMS
Jan 18, 2006·Biochemistry·Jie QinLukasz Lebioda
Oct 25, 2006·The American Journal of Hospice & Palliative Care·Karen A Kehl
Oct 5, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Luci M DusseBashir A Lwaleed
Jan 29, 2011·Diabetes Care·David B Sacks
May 28, 2011·Diabetes Care·David B SacksUNKNOWN Evidence-Based Laboratory Medicine Committee of the American Association for Clinical Chemistry
Sep 28, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Raymond Gambino, David E Bruns

❮ Previous
Next ❯

Citations

Feb 18, 2016·BJOG : an International Journal of Obstetrics and Gynaecology·N Daly, M J Turner
Dec 15, 2015·Primary Care Diabetes·Graziella BonettiMariarosa Carta
Nov 2, 2016·Annals of Clinical Biochemistry·Jasmijn A van BalverenRon Kusters
Mar 17, 2017·Clinical Biochemistry·Sara PasqualettiMauro Panteghini
Mar 12, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Theresa WinterAstrid Petersmann
Oct 31, 2018·Journal of Animal Physiology and Animal Nutrition·Florian Karl ZeugswetterIlse Schwendenwein
Jul 1, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Eline A E van der HagenSjoerd A A van den Berg
Sep 10, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Theresa WinterAstrid Petersmann
Feb 16, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Sjoerd A A van den BergWouter M Tiel Groenestege
Mar 12, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Eline A E van der HagenRobbert J Slingerland
Nov 20, 2019·International Journal of Environmental Research and Public Health·Emma L JamiesonJulia V Marley
Dec 17, 2019·Biochemia Medica·Isabel García-Del-PinoRubén Gómez-Rioja
Feb 20, 2020·Indian Journal of Clinical Biochemistry : IJCB·Preethi LoganathanSelvakumar Kandaswamy
Oct 28, 2019·Practical Laboratory Medicine·Matthias OrthLieselotte Lennartz
Jan 27, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Sjoerd A A van den BergMarc H M Thelen
Oct 16, 2020·Diabetic Medicine : a Journal of the British Diabetic Association·M CartaD Giavarina
Aug 28, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Dominika SzokeMauro Panteghini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Annals of Clinical Biochemistry
C J Pearce, M P Bosomworth
Clinical Chemistry and Laboratory Medicine : CCLM
Yolandie HaydenJohannes M Kuyl
© 2022 Meta ULC. All rights reserved