Iterative cycle of widely targeted metabolic profiling for the improvement of 1-butanol titer and productivity in Synechococcus elongatus

Biotechnology for Biofuels
Artnice Mega FathimaEiichiro Fukusaki

Abstract

Metabolomics is the comprehensive study of metabolites that can demonstrate the downstream effects of gene and protein regulation, arguably representing the closest correlation with phenotypic features. Hence, metabolomics-driven approach offers an effective way to facilitate strain improvement. Previously, targeted metabolomics on the 1-butanol-producing cyanobacterial strain Synechococcus elongatus BUOHSE has revealed the reduction step from butanoyl-CoA to butanal, catalyzed by CoA-acylating propionaldehyde dehydrogenase (PduP), as a rate-limiting step in the CoA-dependent pathway. Moreover, an increase in acetyl-CoA synthesis rate was also observed in this strain, by which the increased rate of release of CoA from butanoyl-CoA was used to enhance formation of acetyl-CoA to feed into the pathway. In the present study, a new strain (DC7) with an improved activity of PduP enzyme, was constructed using BUOHSE as the background strain. DC7 showed a 33% increase in 1-butanol production compared to BUOHSE. For a deeper understanding of the metabolic state of DC7, widely targeted metabolomics approach using ion-pair reversed-phase LC/MS was performed. Results showed a decreased level of butanoyl-CoA and an increased level of acetyl...Continue Reading

References

Oct 19, 2007·Metabolic Engineering·Shota AtsumiJames C Liao
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Qiang HuAl Darzins
May 26, 2009·Metabolic Engineering·David R NielsenKristala L Jones Prather
Jun 30, 2009·Metabolic Engineering·Wenjuan ZhaHuimin Zhao
Oct 6, 2009·Nature Biotechnology·Howard M SalisChristopher A Voigt
Mar 3, 2010·Applied Microbiology and Biotechnology·Oksana V BerezinaVladimir V Zverlov
May 17, 2011·Metabolic Engineering·Ethan I Lan, James C Liao
May 24, 2011·Methods in Enzymology·Howard M Salis
Jun 3, 2011·The Journal of Biological Chemistry·Zhentian LeiLloyd W Sumner
Jan 28, 2012·Journal of Bioscience and Bioengineering·Hiroko KatoAkihiko Kondo
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ethan I Lan, James C Liao
Jul 7, 2012·Biotechnology and Bioengineering·Yu-Sin JangSang Yup Lee
Feb 2, 2013·Journal of Bioscience and Bioengineering·Sastia P PutriEiichiro Fukusaki
Sep 7, 2013·Frontiers in Microbiology·Bertram M BerlaHimadri B Pakrasi
Jan 15, 2014·Metabolic Engineering·John W K OliverShota Atsumi
Aug 26, 2014·Frontiers in Bioengineering and Biotechnology·Anne M Ruffing
Oct 5, 2014·Applied Microbiology and Biotechnology·Seong-Hee ParkJi-Sook Hahn
Mar 4, 2015·Metabolic Engineering·Kangjian QiaoGregory Stephanopoulos
Jan 15, 2016·Metabolomics : Official Journal of the Metabolomic Society·Shingo NoguchiEiichiro Fukusaki
Mar 31, 2016·Nature Reviews. Microbiology·James C LiaoShanshan Luo
Sep 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Peng XuGregory Stephanopoulos
Apr 25, 2018·Metabolic Engineering·Sammy PontrelliJames C Liao

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Citations

Feb 23, 2020·Frontiers in Bioengineering and Biotechnology·Birthe HalmschlagLars M Blank
Sep 23, 2019·Journal of Bioscience and Bioengineering·Artnice Mega FathimaEiichiro Fukusaki
Jun 18, 2020·Journal of Bioscience and Bioengineering·Birthe HalmschlagLars Mathias Blank
Sep 7, 2021·World Journal of Microbiology & Biotechnology·Pengfei GuQiang Li
Oct 26, 2021·Current Opinion in Biotechnology·Damini JaiswalPramod P Wangikar

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Methods Mentioned

BETA
PCA
genetic modification
PCR
reverse transcription PCR

Software Mentioned

MS Excel
P +
MRMPROBS
Primer
SIMCA
Salis calculator
Salis RBS calculator
CFX Manager
Blast

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