iTRAQ-based analysis of changes in the cassava root proteome reveals pathways associated with post-harvest physiological deterioration

The Plant Journal : for Cell and Molecular Biology
Judith OwitiHervé Vanderschuren


The short storage life of harvested cassava roots is an important constraint that limits the full potential of cassava as a commercial food crop in developing countries. We investigated the molecular changes during physiological deterioration of cassava root after harvesting using isobaric tags for relative and absolute quantification (iTRAQ) of proteins in soluble and non-soluble fractions prepared during a 96 h post-harvest time course. Combining bioinformatic approaches to reduce information redundancy for unsequenced or partially sequenced plant species, we established a comprehensive proteome map of the cassava root and identified quantitatively regulated proteins. Up-regulation of several key proteins confirmed that physiological deterioration of cassava root after harvesting is an active process, with 67 and 170 proteins, respectively, being up-regulated early and later after harvesting. This included regulated proteins that had not previously been associated with physiological deterioration after harvesting, such as linamarase, glutamic acid-rich protein, hydroxycinnamoyl transferase, glycine-rich RNA binding protein, β-1,3-glucanase, pectin methylesterase, maturase K, dehydroascorbate reductase, allene oxide cyclase, a...Continue Reading


Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S Karlin, S F Altschul
Mar 25, 1981·Journal of Molecular Biology·T F Smith, M S Waterman
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·S RavanelR Douce
May 9, 2002·Journal of Experimental Botany·Shigeru ShigeokaKazuya Yoshimura
Aug 15, 2002·Plant Physiology·María Angélica SantanaRafael Rangel Aldao
Jun 5, 2003·Plant Molecular Biology·Irene StenzelClaus Wasternack
Jul 15, 2003·Plant Physiology·Hong-Tao LiuRen-Gang Zhou
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Raymond E. Zielinski
Apr 1, 2004·The Plant Journal : for Cell and Molecular Biology·Elisabeth ChanliaudMichael J Gidley
Jul 13, 2004·Plant Physiology·Maike C Rentel, Marc R Knight
Sep 24, 2004·Molecular & Cellular Proteomics : MCP·Philip L RossDarryl J Pappin
Jan 27, 2005·Plant Molecular Biology·Kim ReillyJohn R Beeching
May 3, 2005·Annual Review of Plant Biology·Nicolas BouchéHillel Fromm
Nov 2, 2005·Nature Reviews. Molecular Cell Biology·Daniel J Cosgrove
Dec 20, 2005·Trends in Plant Science·Ron Mittler
Mar 23, 2006·Journal of Experimental Botany·Matthias BergLennart Björkesten
Apr 1, 2006·Journal of Experimental Botany·Stefanie Wienkoop, Wolfram Weckwerth
Apr 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tom P J DunkleyKathryn S Lilley
Aug 1, 2006·Current Opinion in Plant Biology·Bastiaan O Bargmann, Teun Munnik
May 15, 2007·Trends in Plant Science·Jérôme PellouxEwa J Mellerowicz
Jul 12, 2007·Proteomics·Ming ChenXiaohong Qian
Jul 20, 2007·Proteomics·Ghasem Hosseini Salekdeh, Setsuko Komatsu
Dec 7, 2007·Journal of Proteome Research·Lukas KällWilliam Stafford Noble
Mar 22, 2008·Current Opinion in Cell Biology·Satoshi Yamasaki, Paul Anderson
Apr 11, 2008·The Journal of Biological Chemistry·Gaetan GlauserJean-Luc Wolfender
Dec 25, 2008·Molecular & Cellular Proteomics : MCP·Mengmeng ZhuSixue Chen

❮ Previous
Next ❯


Nov 9, 2015·Journal of Plant Research·Maliwan NaconsieJarunya Narangajavana
May 19, 2012·Journal of Proteome Research·Gwenael PottiezPawel Ciborowski
Dec 30, 2014·Plant Biotechnology Journal·Huibin XuJianfu Zhang
Mar 17, 2016·Journal of Agricultural and Food Chemistry·Gabriela Justamante Hendel SchmitzJoão Roberto Oliveira Nascimento
Nov 4, 2016·Plant Cell Reports·Marimuthu KumaravelKallu Purayil Sajith
Dec 10, 2017·Scientific Reports·Ratana ThanasomboonTreenut Saithong
Aug 31, 2017·Molecular Genetics and Genomics : MGG·Zhi-Jun WuJing Zhuang
Mar 14, 2013·Proteomics·Romina PedreschiErnst Woltering
Feb 25, 2017·Journal of the Science of Food and Agriculture·Shubo LiMouming Zhao
Apr 18, 2020·Scientific Reports·Ratana ThanasomboonTreenut Saithong
Nov 25, 2016·Plant Molecular Biology Reporter·Feifei AnSongbi Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.