iTRAQ-based quantitative proteomic analysis of alterations in the intestine of Hu sheep under weaning stress

PloS One
Kai CuiQiyu Diao

Abstract

When Lambs are weaned off ewe's milk, metabolic, structural, and functional changes often occur in the small intestine. Because information on the effects of weaning stress on the proteome of the intestine is limited, an animal model was established with eight pairs of twin lambs divided into artificially reared and ewe-reared groups, which was followed by proteome analysis using iTRAQ technology. Changes occurred in the morphology of the intestine and 5,338 proteins in three biological replicates with less than a 1.2% false discovery rate were identified and quantified. Among them, a subset of 389 proteins were screened as significantly up- (143) and down-regulated (246) in artificially reared compared with ewe-reared. According to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, the differentially expressed proteins that were strongly down-regulated were enriched in immune system processes, biological adhesion, and metabolic processes. The up-regulated proteins were enriched in gene expression, cellular biosynthetic processes, ribosome and RNA binding in response to weaning stress. A series of proteins associated with intestine morphology and immune function were identified, and levels of...Continue Reading

References

May 18, 1999·Reproduction, Nutrition, Development·P OrgeurF Lévy
Feb 17, 2001·Applied Animal Behaviour Science·F C. Flower, D M. Weary
May 8, 2001·The Journal of Nutrition·M A SpreeuwenbergM W Verstegen
Dec 5, 2002·Archiv für Tierernährung·Xianhong GuRuiping She
Aug 13, 2003·Current Opinion in Clinical Nutrition and Metabolic Care·Rémy MeierMichael Steuerwald
Jun 5, 2004·Developmental Cell·Ichiko SaotomeAndrea I McClatchey
Aug 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·John A T Young, Andrew Dillin
Mar 18, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Luca MieleAntonio Grieco
Apr 24, 2009·Vaccine·Michael C Carroll
Feb 6, 2010·Physiology·Steve TerryMaria S Balda
Jan 14, 2012·Current Opinion in Gastroenterology·Rebeca Santaolalla, Maria T Abreu
May 29, 2012·Journal of Proteomics·Peter David EckersallIngrid Miller
Mar 26, 2013·Research in Veterinary Science·Wageha A AwadJürgen Zentek
May 2, 2013·Journal of Animal Science and Biotechnology·Joy M CampbellJavier Polo
Aug 12, 2014·Journal of Applied Animal Welfare Science : JAAWS·María Pascual-AlonsoGustavo A Maria
Sep 10, 2014·Functional & Integrative Genomics·Lorenzo BombaPaolo Ajmone-Marsan
Nov 5, 2014·Genetics and Molecular Research : GMR·P-F HuY-H Ma

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Citations

Dec 2, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Jared DeyarminJane Khudyakov

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Methods Mentioned

BETA
Protein Assay
PCR

Software Mentioned

Blast2GO
Mascot
Proteome Discoverer
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