iTRAQ-based quantitative proteomic analysis reveals the distinct early embryo myofiber type characteristics involved in landrace and miniature pig

BMC Genomics
Xumeng ZhangDelin Mo

Abstract

Pig (Sus scrofa) is a major source of dietary proteins for human consumption and is becoming a valuable model in agricultural and biomedical research. The recently developed isobaric tag for relative and absolute quantitation (iTRAQ) method allows sensitive and accurate protein quantification. Here, we performed the first iTRAQ-based quantitative proteomic analyses of Landrace (LR) and Wuzhishan (WZS) pig longissimus dorsi muscle tissues during early embryonic development. The iTRAQ-based early embryonic longissimus dorsi muscle study of LR and WZS ranging from 21 to 42 days post coitus (dpc) identified a total of 4431 proteins from 17,214 peptides, which were matched with 36,4025 spectra at a false discovery rate of 5%. In both WZS and LR, the largest amount of differentially expressed proteins (DEPs) were found between 28 and 35 dpc. 252 breed-DEPs were selected by GO analysis, including 8 myofibrillar proteins. Only MYHCI/IIA mRNA were detected due to early embryonic stages, and significantly higher expression of them were found in WZS during these 4 stages. MYHCI was first found in WZS at 28 dpc and expressed in both breeds at later stages, while MYHCII protein was not detected until 35 dpc in both breeds. Thus, 33 myogenic...Continue Reading

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Citations

Aug 25, 2017·International Journal of Molecular Sciences·Long ZhangYuling Li
May 31, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xumeng ZhangDelin Mo
Nov 9, 2018·Nature Communications·Manu TamminenAnita Narwani
May 24, 2017·Frontiers in Physiology·Hongjia OuyangQinghua Nie
Aug 15, 2019·Animals : an Open Access Journal From MDPI·Ji-Wei LiPei-Shi Yan
Mar 24, 2021·Journal of Agricultural and Food Chemistry·Aiwen JiangHonglin Liu

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Datasets Mentioned

BETA
PXD002481

Methods Mentioned

BETA
PCR

Software Mentioned

STRING
DAVID
Ensembl
Blast2GO
iTRAQ
Mascot
Cytoscape

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