iTRAQ quantitative analysis of multidrug resistance mechanisms in human gastric cancer cells.

Journal of Biomedicine & Biotechnology
Huai-Dong HuSang-Lin Li

Abstract

Multidrug resistance (MDR) is a major obstacle towards a successful treatment of gastric cancer. However, the mechanisms of MDR are intricate and have not been fully understood. To elucidate the molecular mechanisms of MDR in gastric cancer, we employed the proteomic approach of isobaric tags for relative and absolute quantification (iTRAQ), followed by LC-MS/MS, using the vincristine-resistant SGC7901/VCR cell line and its parental SGC7901 cell line as a model. In total, 820 unique proteins were identified and 91 proteins showed to be differentially expressed in SGC7901/VCR compared with SGC7901. Several differentially expressed proteins were further validated by western blot analysis. Furthermore, the association of MVP, one of the highly expressed proteins in SGC7901/VCR, with MDR was verified. Our study is the first application of iTRAQ technology for MDR mechanisms analysis in gastric cancer, and many of the differentially expressed proteins identified have not been linked to MDR in gastric cancer before, which showed the value of this technology in identifying differentially expressed proteins in cancer.

References

Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·I G WoolA Glück
Jan 1, 1996·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·M Dietel
May 16, 1998·The Journal of Biological Chemistry·V A KickhoeferL H Rome
Jul 11, 1998·Molecular Cell·H HermekingB Vogelstein
Oct 8, 1999·International Journal of Urology : Official Journal of the Japanese Urological Association·S NaitoH Koga
Sep 13, 2000·Nature·S Lloyd
Jan 10, 2001·International Journal of Cancer. Journal International Du Cancer·M KitazonoS Akiyama
Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·A C SivaL H Rome
Oct 3, 2003·Cellular and Molecular Life Sciences : CMLS·A van ZonE A C Wiemer
Mar 12, 2005·CA: a Cancer Journal for Clinicians·D Max ParkinPaola Pisani
Nov 16, 2005·International Journal of Cancer. Journal International Du Cancer·Jozien HellemanEls M J J Berns
Mar 31, 2007·Molecular Carcinogenesis·Wenqing QiJesse D Martinez
Jun 20, 2007·Photochemistry and Photobiology·Nicholas E DickensonRobert C Dunn
Oct 20, 2007·Expert Review of Anticancer Therapy·Dexin Zhang, Daiming Fan
Feb 7, 2008·Journal of Cellular Biochemistry·Yi-Xuan YangZhi-Qiang Xiao
Mar 29, 2008·Methods in Molecular Biology·Walter Weiss, Angelika Görg
Nov 18, 2008·Biochemical and Biophysical Research Communications·Berit R HøjMartin W Berchtold
Feb 26, 2009·Methods in Molecular Biology·Alexander Leitner, Wolfgang Lindner
May 15, 2009·Electrophoresis·Angelika GörgWalter Weiss
May 16, 2009·Anti-cancer Agents in Medicinal Chemistry·Christiane Baumert, Andreas Hilgeroth
Aug 18, 2009·Expert Review of Proteomics·Lianghai HuHanfa Zou

❮ Previous
Next ❯

Citations

Feb 14, 2014·Leukemia Research·Mohammad Reza Noori-DaloiiAli Reza Noori-Daloii
Nov 30, 2012·Journal of Biomedicine & Biotechnology·Katerina CizkovaJiri Ehrmann
Mar 13, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xinwei ZhaoJinyong Peng
Jun 17, 2011·Journal of Cellular Biochemistry·Feng YeJian-Bin Tong
Jul 8, 2016·Scandinavian Journal of Immunology·D D HouH Q Guan
Jan 18, 2014·International Journal of Molecular Medicine·Ken-ichi MatsumotoTeiji Oda
Jan 16, 2020·International Journal of Molecular Sciences·Teodora CosteaMaria-Magdalena Mocanu
Sep 17, 2011·Medicinal Research Reviews·Frederick HarrisDavid A Phoenix
Jun 27, 2019·Journal of Cellular Biochemistry·Xianjun SunJingcheng Dong

❮ Previous
Next ❯

Datasets Mentioned

BETA
SGC7901

Methods Mentioned

BETA
PCR
transfection

Software Mentioned

PANTHER
ProteinPilot

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.