iTRAQ quantitative proteomics-based identification of cell adhesion as a dominant phenotypic modulation in thrombin-stimulated human aortic endothelial cells

Thrombosis Research
Huang-Joe WangWan-Yu Lo

Abstract

The phenotypic changes in thrombin-stimulated endothelial cells include alterations in permeability, cell shape, vasomotor tone, leukocyte trafficking, migration, proliferation, and angiogenesis. Previous studies regarding the pleotropic effects of thrombin on the endothelium used human umbilical vein endothelial cells (HUVECs)-cells derived from fetal tissue that does not exist in adults. Only a few groups have used screening approaches such as microarrays to profile the global effects of thrombin on endothelial cells. Moreover, the proteomic changes of thrombin-stimulated human aortic endothelial cells (HAECs) have not been elucidated. HAECs were stimulated with 2 units/mL thrombin for 5h and their proteome was investigated using isobaric tags for the relative and absolute quantification (iTRAQ) and the MetaCore(TM) software. A total of 627 (experiment A) and 622 proteins (experiment B) were quantified in the duplicated iTRAQ analyses. MetaCore(TM) pathway analysis identified cell adhesion as a dominant phenotype in thrombin-stimulated HAECs. Replicated iTRAQ data revealed that "Cell adhesion_Chemokines and adhesion," "Cell adhesion_Histamine H1 receptor signaling in the interruption of cell barrier integrity," and "Cell adhe...Continue Reading

References

May 11, 2002·Critical Care Medicine·Marcel LeviTom van der Poll
May 16, 2003·Proteomics·Arnaud BruneelBruno Baudin
May 20, 2003·The Journal of Biological Chemistry·Corinne PellieuxPaolo Silacci
Mar 27, 2004·Physiological Reviews·Valeria S Ossovskaya, Nigel W Bunnett
Sep 2, 2004·The Journal of Biological Chemistry·Unni M ChandrasekharanPaul E DiCorleto
Apr 9, 2005·The Journal of Biological Chemistry·Joseph N McLaughlinHeidi E Hamm
Dec 17, 2005·Thrombosis Research·Makoto OkadaTadashi Shinohara
Jun 15, 2006·Briefings in Functional Genomics & Proteomics·Kunal AggarwalKelvin H Lee
Mar 31, 2007·Circulation Research·Laurent LamaliceJacques Huot
Aug 8, 2007·Journal of Vascular Surgery·Peter B Brant-ZawadzkiLarry W Kraiss
Dec 11, 2007·Journal of Proteome Research·Lukas KällWilliam Stafford Noble
Apr 1, 2008·Annals of the New York Academy of Sciences·Emily VandenbrouckeAsrar B Malik
Mar 7, 2012·Current Pharmaceutical Design·Antonino TuttolomondoGiuseppe Licata
Dec 19, 2012·Biochemical and Biophysical Research Communications·Huang-Joe WangLi-Jen Lin
Jun 28, 2013·Cytoskeleton·Hannah SchachtnerLaura M Machesky
Jul 28, 2013·Immunobiology·Ioana Madalina Fenyo, Anca Violeta Gafencu

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