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IVT-seq reveals extreme bias in RNA sequencing

Genome Biology

Jun 30, 2014

Nicholas F. LahensJohn B. Hogenesch

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Abstract

RNA-seq is a powerful technique for identifying and quantifying transcription and splicing events, both known and novel. However, given its recent development and the proliferation of library construction methods, understanding the bias it introduces is incomplete but critical to realiz...read more

Mentioned in this Paper

15S RNA
Exons
Mice, Inbred C57BL
RNA
RNA Splicing
Ribosomal RNA
Transcription, Genetic
Morphologic Artifacts
DNA Sequence
Homologous Sequences, Nucleic Acid
124
427
1
2
Paper Details
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  • IVT-seq reveals extreme bias in RNA sequencing

    Genome Biology

    Jun 30, 2014

    Nicholas F. LahensJohn B. Hogenesch

    PMID: 24981968

    DOI: 10.1186/gb-2014-15-6-r86

    Abstract

    RNA-seq is a powerful technique for identifying and quantifying transcription and splicing events, both known and novel. However, given its recent development and the proliferation of library construction methods, understanding the bias it introduces is incomplete but critical to realiz...read more

    Mentioned in this Paper

    15S RNA
    Exons
    Mice, Inbred C57BL
    RNA
    RNA Splicing
    124
    427
    1
    2
    Paper Details
    References
    • References
    • Citations38

      References currently unavailable

      We're still populating references for this paper, please check back later.
    • References
    • Citations38
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