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IVT-seq reveals extreme bias in RNA sequencing

Genome Biology

Jul 2, 2014

Nicholas LahensJohn B Hogenesch

Abstract

RNA-seq is a powerful technique for identifying and quantifying transcription and splicing events, both known and novel. However, given its recent development and the proliferation of library construction methods, understanding the bias it introduces is incomplete but critical to realiz...read more

Mentioned in this Paper

Exons
Sequence Determinations, RNA
Transcription, Genetic
Ribosomal RNA
Morphologic Artifacts
Sequencing
Nuclear mRNA Cis Splicing, via Spliceosome
Homologous Sequences, Nucleic Acid
RNA Splicing
15S RNA
123
429
1
2
Paper Details
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IVT-seq reveals extreme bias in RNA sequencing

Genome Biology

Jul 2, 2014

Nicholas LahensJohn B Hogenesch

PMID: 24981968

DOI: 10.1186/gb-2014-15-6-r86

Abstract

RNA-seq is a powerful technique for identifying and quantifying transcription and splicing events, both known and novel. However, given its recent development and the proliferation of library construction methods, understanding the bias it introduces is incomplete but critical to realiz...read more

Mentioned in this Paper

Exons
Sequence Determinations, RNA
Transcription, Genetic
Ribosomal RNA
Morphologic Artifacts
Sequencing
Nuclear mRNA Cis Splicing, via Spliceosome
Homologous Sequences, Nucleic Acid
RNA Splicing
15S RNA
123
429
1
2

Similar Papers Found In These Feeds

RNA Splicing (Keystone)

The majority of human genes that encode proteins undergo alternative pre-mRNA splicing and mutations that affect splicing are more prevalent than previously thought. Discover the latest research on RNA splicing and co-transcriptional splicing in this feed.

Exon Skipping

Exon skipping is a method of RNA splicing used when a mutation occurs in the genes of exons. It is regulated largely by the use of antisense oligonucleotides that mask the mutated gene and allow the cellular machinery to skip over it. Exon skipping is being investigated as a therapeutic option for some diseases. Here is the latest research on exon skipping.

Related Papers

BMC Genomics

RNA-Seq optimization with eQTL gold standards

BMC GenomicsDecember 18, 2013
Shannon E EllisDan E Arking
Paper Details
References
  • References
  • Citations42
  • finger pointing at paper

    References currently unavailable

    We're still populating references for this paper, please check back later.
  • References
  • Citations42
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