PMID: 9541574Apr 29, 1998Paper

Jak-STAT pathway is involved in the induction of TNF-beta gene during stimulation by IL-2

European Journal of Immunology
L LuX Liu

Abstract

IL-2 is the major regulatory cytokine of the immune system. It plays a key role in T cell survival, growth and activation. IL-2 may induce the expression of multiple genes including some cytokine genes. The induction of these genes is triggered by different signal pathways, one of them being the Jak-STAT signal pathway. The genes regulated by this pathway remain to be determined. By studying IL-2-inducible genes, we have confirmed that the TNF-beta gene is one of the immediate early genes activated by IL-2. By analysis of the DNA sequences around 180-300 bases upstream of the transcription initiation point of the mouse TNF-beta gene, we demonstrate that there is a STAT5 binding site which is essential to the inducibility of the TNF-beta gene. Furthermore, in BA/F3 cells co-transfected with the STAT5A gene and IL-2R beta gene, the activation of the TNF-beta gene promoter by IL-2 was greatly promoted, whereas the TNF-beta gene promoter became IL-2-non-inducible if the STAT5A gene was substituted with a dominant negative STAT5A, i.e. a C-terminally truncated mutant. Taken together, our results show that the Jak-STAT signal pathway is involved in induction of the TNF-beta gene in cells stimulated by IL-2.

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Citations

Mar 29, 2000·Biochemical and Biophysical Research Communications·H JiZ Zheng
May 9, 2001·Biochemical and Biophysical Research Communications·W TangX Liu
Dec 14, 2001·Biochemical and Biophysical Research Communications·W XuX Liu
Jan 27, 2004·Biochemical and Biophysical Research Communications·Zhangfei ShouKaoru Miyamoto
Dec 1, 2000·Biochemical and Biophysical Research Communications·M YanZ Zheng
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Di WangLinrong Lu
Dec 13, 2017·Journal of Immunotoxicology·Francisco Gallardo-VeraErika P Rendón-Huerta
Aug 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·T KubotaJ M Krueger
Apr 8, 2004·Physiological Genomics·Jean F ChallacombeThomas S Brettin
Aug 30, 2008·Science in China. Series C, Life Sciences·J ZhuX Liu
Apr 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Steven J BensingerLaurence A Turka
Jan 8, 2015·Journal of Immunotoxicology·Francisco Gallardo-VeraLuis F Montaño
Jun 16, 2006·Biology of Reproduction·Makoto NakasatoFugaku Aoki
May 30, 2019·Frontiers in Oncology·Qingqing CaiKen H Young
Jun 19, 2012·Cancer Discovery·Ghee Chong KooSoon Thye Lim

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