JAK2V617F/STAT5 signaling pathway promotes cell proliferation through activation of Pituitary Tumor Transforming Gene 1 expression

Biochemical and Biophysical Research Communications
Xu-Liang ShenFang-Ping Chen

Abstract

Gain-of-function mutations of JAK2 play crucial roles in the development of myeloproliferative neoplasms; however, the underlying downstream events of this activated signaling pathway are not fully understood. Our experiment was designed and performed to address one aspect of this issue. Here we report that AG490, a potent JAK2V617F kinase inhibitor, effectively inhibits the proliferation of HEL cells. Interestingly, AG490 also decreases the expression of PTTG1, a possible target gene of the aberrant signaling pathway, in a dose- and time-dependent manner. Furthermore, the promoter activity analyses reveal that the inhibition of the PTTG1 expression is affected at the transcriptional level. Thus, our results suggest that the JAK2V617F/STAT5 signaling pathway promotes cell proliferation through the transcriptional activation of PTTG1.

References

Apr 25, 2000·Molecular and Cellular Biology·P SaharinenO Silvennoinen
Apr 29, 2005·The New England Journal of Medicine·Robert KralovicsRadek C Skoda
May 3, 2005·The Journal of Biological Chemistry·Runxiang ZhaoZhizhuang Joe Zhao
Apr 25, 2006·The Journal of Biological Chemistry·Christoph WalzMartin Sattler
Feb 28, 2007·Endocrine Reviews·George VlotidesShlomo Melmed
Sep 8, 2007·American Journal of Nephrology·Rong LiXueqing Yu
Aug 30, 2008·Endocrine-related Cancer·Fateme SalehiMichael Cusimano
May 30, 2009·Molecular Biology of the Cell·Agueda G EspinaJosé A Pintor-Toro
Mar 2, 2010·International Journal of Hematology·Yasumichi HitoshiVadim Markovtsov
Mar 2, 2010·International Journal of Hematology·Martha Wadleigh, Ayalew Tefferi
Apr 22, 2010·Cancer Research·Rachel J WatkinsChristopher J McCabe

❮ Previous
Next ❯

Citations

Aug 9, 2012·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S Q HuangG Ye
Feb 25, 2014·The Journal of Membrane Biology·Zohreh HosseinzadehFlorian Lang
Mar 13, 2012·Biochemical and Biophysical Research Communications·Zohreh HosseinzadehFlorian Lang
Jan 15, 2013·Biochemical and Biophysical Research Communications·Manzar ShojaiefardFlorian Lang
Dec 5, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zohreh HosseinzadehFlorian Lang
Apr 4, 2014·American Journal of Physiology. Cell Physiology·Zohreh HosseinzadehFlorian Lang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis