JAK/STAT3 signalling is sufficient and dominant over antagonistic cues for the establishment of naive pluripotency.

Nature Communications
Anouk L van OostenJosé C R Silva

Abstract

Induced pluripotency depends on cooperativity between expression of defined factors and the culture environment. The latter also determines the pluripotent cell state, that is, naïve or primed. LIF-JAK/STAT3 signalling was recently shown to be a limiting factor for reprogramming to naïve pluripotency. Here we show that sufficient activation of JAK/STAT3 overcomes the reprogramming block of cell intermediates and enables somatic cell reprogramming in absence of otherwise essential pluripotency medium requisites. Activation of FGF-ERK signalling, which promotes exit of naïve pluripotent cells from self-renewal, does not prevent JAK/STAT3 induced post-implantation epiblast-derived stem cell conversion into naïve pluripotency. Moreover, even in the presence of FGF plus Activin, which instructs and maintains the primed state, JAK/STAT3 enforces naïve pluripotency in epiblast stem cells. We conclude that JAK/STAT3 signalling can be sufficient and dominant over antagonistic cues to enable the induction of a naïve pluripotent s...Continue Reading

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Citations

Sep 21, 2013·Stem Cell Reports·Harry G LeitchAustin Smith
Jan 4, 2013·Stem Cells and Development·Vinod VermaBjörn Oback
Apr 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yohei HayashiRobert J Fletterick
Aug 26, 2015·Stem Cells·Chen-Yun ChenPatrick C H Hsieh
Feb 18, 2016·Stem Cells International·Alastair H Davies, Amina Zoubeidi
Jan 28, 2014·Current Biology : CB·Hannah T StuartJosé C R Silva
Aug 13, 2013·Current Opinion in Genetics & Development·Nicola FestucciaIan Chambers
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Jun 23, 2015·Nature Biotechnology·Ori Bar-NurKonrad Hochedlinger
Jan 29, 2014·JAK-STAT·Yong Tang, Xiuchun Cindy Tian
Sep 1, 2014·Current Opinion in Genetics & Development·Shoudong YeQi-Long Ying
Jan 18, 2015·Cellular and Molecular Life Sciences : CMLS·Guanyi HuangQi-Long Ying
Feb 24, 2017·Journal of Cellular Biochemistry·Na LiJinlian Hua
Apr 18, 2018·Nature Cell Biology·Lydia W S FinleyCraig B Thompson
Feb 26, 2015·The EMBO Journal·Wataru Ebina, Derrick J Rossi

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