JAM-A knockdown accelerates the proliferation and migration of human keratinocytes, and improves wound healing in rats via FAK/Erk signaling

Cell Death & Disease
Yunchuan WangXiaobing Fu

Abstract

Junctional adhesion molecule-A (JAM-A) belongs to the immunoglobulin superfamily, it predominantly exists at the tight junctions of epithelial and endothelial cells. JAM-A is known to regulate leukocyte trans-endothelial migration, however, how it affects the proliferation and migration of keratinocytes, the two essential steps during wound healing, has less been explored. In this study, we showed that JAM-A was significantly expressed in normal skin epidermis. RNAi-mediated JAM-A knockdown remarkably promoted the proliferation and migration of keratinocytes. We also found that loss of JAM-A increased the protein levels of p-FAK, p-Erk1/2, and p-JNK; however, FAK inhibitor PF-562271 restrained the expression of p-FAK and p-Erk1/2 elevated by JAM-A RNAi, but not p-JNK, and also slowed down keratinocyte proliferation and migration. Finally, in a rat wound model we showed that absence of JAM-A significantly promoted the wound healing process, while the use of PF-562271 or Erk1/2 inhibitor PD98059 repressed those effects. These data collectively demonstrate that suppressing JAM-A expression could promote the proliferation and migration of keratinocytes and accelerate the healing process of rat skin wounds, potentially via FAK/Erk p...Continue Reading

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Citations

Mar 19, 2019·Journal of Cellular Biochemistry·Peng WuYibing Wang
May 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kyoung-Ok HongSeong Doo Hong
Mar 30, 2021·International Journal of Rheumatic Diseases·Tzu-Jung FangJeng-Hsien Yen
Apr 3, 2020·Biochimica Et Biophysica Acta. Biomembranes·Lorenza González-MariscalElida Amaya
Jul 7, 2021·Cell Death and Differentiation·Swati Mohapatra, George Calin

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Methods Mentioned

BETA
transfection
PCR
xenograft

Software Mentioned

Image
SPSS
Pro Plus

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