Janus Liposomes: Gel-Assisted Formation and Bioaffinity-Directed Clustering

Langmuir : the ACS Journal of Surfaces and Colloids
Mingming WangWei Zhan

Abstract

This article reports a high-yield procedure for preparing microsized (giant) Janus liposomes via gel-assisted lipid swelling and clustering behavior of these liposomes directed by biotin-avidin affinity binding. Confocal fluorescence microscopy reveals in detail that these new lipid colloidal particles display broken symmetry and heterogeneous surface chemistry similar to other types of Janus particles. An optimized formation procedure is presented, which reproducibly yields large liposome populations dominated by a single-domain configuration. This work further demonstrates that biotin-conjugated 1,2-dioleoyl- sn-glycero-3-phosphoethanolamine preferentially partitions into the liquid-disordered phase of the lipid matrix, rendering these Janus liposomes asymmetrical binding capacity toward avidin. This affinity binding drives irreversible and domain-specific cluster formation among Janus liposomes, whose structure and size are found to depend on the domain configuration of individual liposomes and incubation time.

References

Nov 1, 1973·Zeitschrift Für Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie·W Helfrich
Jul 24, 1998·Trends in Biotechnology·D D Lasic
May 10, 1993·Physical Review Letters·F Jülicher, R Lipowsky
Aug 31, 1999·Biophysical Journal·A Radhakrishnan, H M McConnell
Jan 11, 2000·International Journal of Food Sciences and Nutrition·B F GibbsC N Mulligan
Feb 27, 2001·Biophysical Journal·C DietrichE Gratton
Nov 6, 2001·Progress in Lipid Research·Henna Ohvo-RekiläJ Peter Slotte
Feb 8, 2003·Annual Review of Biophysics and Biomolecular Structure·Harden M McConnell, Marija Vrljic
Jan 1, 1963·Advances in Lipid Research·A D BANGHAM
Jan 28, 2004·Cell·Juan S Bonifacino, Benjamin S Glick
Apr 28, 2004·Biophysical Journal·S L VeatchS L Keller
May 17, 2005·Biophysical Journal·T BaumgartJ T Jenkins
Dec 21, 2005·Nature Reviews. Molecular Cell Biology·Joshua Zimmerberg, Michael M Kozlov
Feb 14, 2006·Biochimica Et Biophysica Acta·Paulo F F AlmeidaAnne Hinderliter
Nov 9, 2006·Nano Letters·Liang HongSteve Granick
Jan 5, 2007·Annual Review of Biophysics and Biomolecular Structure·Gerald W Feigenson
Apr 25, 2007·Critical Reviews in Toxicology·Gerhard J NohynekMichael S Roberts
Oct 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sarah L VeatchKlaus Gawrisch
May 27, 2008·Biophysical Journal·Suliana ManleyAlice P Gast
Oct 1, 2008·Biochimica Et Biophysica Acta·Tomasz RógMikko Karttunen
Oct 31, 2008·Talanta·Katie A Edwards, Antje J Baeumner
Jan 27, 2009·Biophysical Journal·James H DavisJanos Juhasz
Apr 20, 2010·Advanced Materials·Shan JiangSteve Granick
Jan 15, 2011·Physical Review Letters·Hong-Ren JiangMasaki Sano
Mar 9, 2011·Chemical Society Reviews·Jianzhong Du, Rachel K O'Reilly
Jan 26, 2012·International Journal of Nanomedicine·Hsin-I Chang, Ming-Kung Yeh
Apr 6, 2013·Chemical Reviews·Andreas Walther, Axel H E Müller
Jul 5, 2013·Biophysical Journal·Andreas WeinbergerCarlos Marques
Oct 6, 2015·Nano Letters·Xing MaSamuel Sánchez

❮ Previous
Next ❯

Citations

Nov 3, 2020·Physical Chemistry Chemical Physics : PCCP·Shanlong LiYongfeng Zhou
Feb 5, 2019·ACS Applied Materials & Interfaces·Alina KirillovaAlla Synytska
Jul 6, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Vaishnavi GirishAnand Bala Subramaniam
Oct 31, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Zening LiuWei Zhan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved