Jasmonate-responsive expression of paclitaxel biosynthesis genes in Taxus cuspidata cultured cells is negatively regulated by the bHLH transcription factors TcJAMYC1, TcJAMYC2, and TcJAMYC4

Frontiers in Plant Science
Sangram K LenkaElsbeth L Walker

Abstract

Taxus cell suspension culture is a sustainable technology for the industrial production of paclitaxel (Taxol®), a highly modified diterpene anti-cancer agent. The methyl jasmonate (MJ)-mediated paclitaxel biosynthetic pathway is not fully characterized, making metabolic engineering efforts difficult. Here, promoters of seven genes (TASY, T5αH, DBAT, DBBT, PAM, BAPT, and DBTNBT), encoding enzymes of the paclitaxel biosynthetic pathway were isolated and used to drive MJ-inducible expression of a GUS reporter construct in transiently transformed Taxus cells, showing that elicitation of paclitaxel production by MJ is regulated at least in part at the level of transcription. The paclitaxel biosynthetic pathway promoters contained a large number of E-box sites (CANNTG), similar to the binding sites for the key MJ-inducible transcription factor AtMYC2 from Arabidopsis thaliana. Three MJ-inducible MYC transcription factors similar to AtMYC2 (TcJAMYC1, TcJAMYC2, and TcJAMYC4) were identified in Taxus. Transcriptional regulation of paclitaxel biosynthetic pathway promoters by transient over expression of TcJAMYC transcription factors indicated a negative rather than positive regulatory role of TcJAMYCs on paclitaxel biosynthetic gene exp...Continue Reading

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Citations

Dec 19, 2015·ACS Chemical Biology·Steven EdgarGregory Stephanopoulos
Aug 17, 2019·Plant & Cell Physiology·Xueying ChenYing-Bo Mao
Mar 27, 2018·Molecular Biotechnology·Sangram K LenkaKailash C Bansal
Apr 25, 2019·International Journal of Molecular Sciences·Yunpeng CuiZhigang Guo
Dec 9, 2016·Journal of Experimental Botany·Jonas GoossensAlain Goossens
Jun 15, 2021·Frontiers in Plant Science·Sunny AhmarKi-Hung Jung
Jul 17, 2021·Nature Plants·Xingyao XiongJianbin Yan
Oct 6, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sagar S AryaJames E Rookes

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Datasets Mentioned

BETA
M36171.1

Methods Mentioned

BETA
PCR
affinity purification
electrophoresis
electrophoretic mobility shift
RNA-seq

Software Mentioned

- HOP
nsensus - De generate H ybrid O ligonucleotide P rimers ( CODE...
CODE
ClustalW
Co

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