Jianpi Jiedu decoction, a traditional Chinese medicine formula, inhibits tumorigenesis, metastasis, and angiogenesis through the mTOR/HIF-1α/VEGF pathway

Journal of Ethnopharmacology
Weijun PengSifang Zhang

Abstract

Traditional Chinese medicine has been utilized for the treatment of cancer. Jianpi Jiedu decoction (JPJD), a traditional Chinese medicine formula, has been used for the treatment of colorectal cancer for decades. However, the underlying molecular mechanistic basis for the effect of JPJD on colorectal cancer is poorly understood. The aim of this study was to identify the effects of JPJD on human colon cancer cells in vitro as well as in vivo and to investigate the mechanistic basis for the anticancer effect of JPJD. The in vitro antitumor activity of JPJD was assessed by MTT assay, flow cytometric analysis, wound-healing assay, transwell assays, and tube formation assays in order to assess cell activity, apoptosis, migration, invasion, and angiogenesis, respectively. The anticancer properties of JPJD in vivo were assessed by immunohistochemistry in a nude mouse xenograft model of HCT116 cells. In addition, the level of mTOR/HIF-1α/VEGF signaling pathway proteins in HCT116 cells and tumor tissue was evaluated by immunoblotting. In vitro, JPJD significantly inhibited colorectal cancer cell lines viability and proliferation. Flow cytometry analysis demonstrated JPJD to induce HCT116 cell apoptosis. Additionally, JPJD effectively su...Continue Reading

Citations

Sep 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mengling YouDan Ji
Oct 12, 2018·International Journal of Molecular Sciences·Stéphane TerrySalem Chouaib
Nov 16, 2019·BMC Complementary and Alternative Medicine·Liling ZengFuping Xu
Apr 18, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Dan MaoSifang Zhang
Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kailong WangHaiyang Yu
Jan 13, 2021·BMC Complementary Medicine and Therapies·Xiaohan ChenJinju Jiang

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