Jingzhaotoxin-X, a gating modifier of Kv4.2 and Kv4.3 potassium channels purified from the venom of the Chinese tarantula Chilobrachys jingzhao

The Journal of Venomous Animals and Toxins Including Tropical Diseases
Meichun DengSongping Liang

Abstract

The tarantula Chilobrachys jingzhao is one of the largest venomous spiders in China. In previous studies, we purified and characterized at least eight peptides from C. jingzhao venom. In this report, we describe the purification and characterization of Jingzhaotoxin-X (JZTX-X), which selectively blocks Kv4.2 and Kv4.3 potassium channels. JZTX-X was purified using a combination of cation-exchange HPLC and reverse-phase HPLC. The amino-acid sequence was determined by automated Edman degradation and confirmed by mass spectrometry (MS). Voltage-gated ion channel currents were recorded in HEK293t cells transiently transfected with a variety of ion channel constructs. In addition, the hyperalgesic activity of JZTX-X and the toxin´s effect on motor function were assessed in mice. JZTX-X contained 31 amino acids, with six cysteine residues that formed three disulfide bonds within an inhibitory cysteine knot (ICK) topology. In whole-cell voltage-clamp experiments, JZTX-X inhibited Kv4.2 and Kv4.3 potassium channels in a concentration- and voltage-dependent manner, without affecting other ion channels (Kv1.1, 1.2, 1.3, 2.1, delayed rectifier potassium channels, high- and low-voltage-activated Ca2+ channels, and voltage-gated sodium chann...Continue Reading

References

Dec 1, 1991·Cellular and Molecular Neurobiology·D J KlumppL H Pinto
May 31, 1996·The Journal of Biological Chemistry·S AddaM I Kotlikoff
Aug 7, 2002·Journal of Molecular Biology·Koh TakeuchiIchio Shimada
Oct 4, 2003·Nature Reviews. Drug Discovery·Richard J Lewis, Maria L Garcia
Apr 7, 2004·Toxicon : Official Journal of the International Society on Toxinology·Pierre Escoubas, Lachlan Rash
Jul 23, 2004·Physiological Reviews·Shari G BirnbaumLaura A Schrader
Nov 19, 2004·The Journal of Biological Chemistry·Yucheng XiaoSongping Liang
Mar 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Vladislav V ZarayskiyMichael J Morales
Apr 8, 2006·Neuron·Hui-Juan HuRobert W Gereau
Jul 18, 2006·Nucleic Acids Research·Alessio CeroniPaolo Frasconi
Dec 13, 2006·Toxicon : Official Journal of the International Society on Toxinology·Xiongzhi ZengSongping Liang
Jul 17, 2007·Toxicon : Official Journal of the International Society on Toxinology·Chunhua YuanSongping Liang
Sep 15, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Li-Ying ChienMeei-Ling Tsaur
Oct 9, 2008·Biochemical Pharmacology·Meichi WangSongping Liang
Oct 14, 2008·Toxicon : Official Journal of the International Society on Toxinology·Meichi WangSongping Liang
Nov 14, 2008·Nature·Frank BosmansKenton J Swartz
Jul 22, 2009·Journal of Molecular and Cellular Cardiology·Noriko Niwa, Jeanne M Nerbonne
Mar 2, 2010·The Journal of Biological Chemistry·Sébastien Dutertre, Richard J Lewis
Dec 14, 2011·Toxicon : Official Journal of the International Society on Toxinology·Chunhua YuanSongping Liang
Dec 19, 2012·Toxicon : Official Journal of the International Society on Toxinology·Huai TaoSongping Liang
Feb 6, 2013·Biochimica Et Biophysica Acta·David J Adams, Géza Berecki
Dec 25, 2013·Handbook of Clinical Neurology·S A M Kularatne, Nimal Senanayake
Jul 11, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tilia Kimm, Bruce P Bean
Jan 2, 2016·Toxicon : Official Journal of the International Society on Toxinology·Huai TaoSongping Liang
Nov 5, 2016·Toxicon : Official Journal of the International Society on Toxinology·Huai TaoSongping Liang
Mar 24, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yen-Ling KuoMeei-Ling Tsaur

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Datasets Mentioned

BETA
AB003587.1
DQ857292.1
AB158469.2
AF026005.1
BC035059.1

Methods Mentioned

BETA
transfection
NMR

Software Mentioned

DS ViewerPro
ESyPred3D
Sigmaplot

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