JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells

Biochimica Et Biophysica Acta
Tetsuya MizutaniShigeru Morikawa

Abstract

Persistence was established after most of the SARS-CoV-infected Vero E6 cells died. RNA of the defective interfering virus was not observed in the persistently infected cells by Northern blot analysis. SARS-CoV diluted to 2 PFU failed to establish persistence, suggesting that some particular viruses in the seed virus did not induce persistent infection. Interestingly, a viral receptor, angiotensin converting enzyme (ACE)-2, was down-regulated in persistently infected cells. G418-selected clones established from parent Vero E6 cells, which were transfected with a plasmid containing the neomycin resistance gene, were infected with SARS-CoV, resulting in a potential cell population capable of persistence in Vero E6 cells. Our previous studies demonstrated that signaling pathways of extracellular signal-related kinase (ERK1/2), c-Jun N-terminal protein kinase (JNK), p38 mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3'-kinase (PI3K)/Akt were activated in SARS-CoV-infected Vero E6 cells. Previous studies also showed that the activation of p38 MAPK by viral infection-induced apoptosis, and a weak activation of Akt was not sufficient to protect from apoptosis. In the present study, we showed that the inhibitors of J...Continue Reading

References

Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M O DiazJ D Rowley
Mar 1, 1966·Proceedings of the Society for Experimental Biology and Medicine·H Diderholm, Z Dinter
May 6, 2003·Science·Marco A MarraRachel L Roper
Nov 19, 2003·Biochemical and Biophysical Research Communications·Runtao HeXuguang Li
Jun 15, 2004·Biochemical and Biophysical Research Communications·Tetsuya MizutaniShigeru Morikawa
Jul 20, 2004·Journal of Medical Virology·Paul K S ChanHo-Keung Ng
Nov 6, 2004·FEBS Letters·Tetsuya MizutaniShigeru Morikawa

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Citations

Feb 6, 2013·Archives of Virology·Liqian ZhuGuangzhi Tong
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Marie A Bogoyevitch, Bostjan Kobe
Feb 7, 2006·FEBS Letters·Tetsuya MizutaniShigeru Morikawa
Oct 30, 2007·Biochimica Et Biophysica Acta·Marie A Bogoyevitch, Peter G Arthur
May 2, 2007·Annals of the New York Academy of Sciences·T Mizutani
Feb 10, 2010·International Journal of Immunogenetics·X WangX Cheng
Oct 5, 2014·Cytotechnology·Mohammad Khalid Parvez, Mohammed Salem Al-Dosari
Feb 21, 2006·FEMS Immunology and Medical Microbiology·Tetsuya MizutaniShigeru Morikawa
Jul 31, 2016·Research in Veterinary Science·S MitomoT Mizutani
Jul 8, 2016·Viruses·To Sing FungDing Xiang Liu
Jun 22, 2019·Annual Review of Microbiology·To Sing Fung, Ding Xiang Liu
Jul 25, 2016·Diseases·Yvonne Xinyi LimDing Xiang Liu
Aug 9, 2020·Cancers·Ishita GuptaAla-Eddin Al Moustafa
Jul 22, 2020·Emerging Microbes & Infections·Sofia AppelbergUjjwal Neogi
Aug 6, 2020·Biological Procedures Online·Reza Zolfaghari EmamehMajid Khoshmirsafa
Nov 24, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Yuxuan WangMinghua Wu
Apr 4, 2021·International Journal of Molecular Sciences·J Michael HarnishShinya Yamamoto
Jul 9, 2021·Journal of Molecular Graphics & Modelling·K Amith KumarPravindra Kumar

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Methods Mentioned

BETA
PCR
transfection

Related Concepts

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AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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