JNK regulates cell migration through promotion of tyrosine phosphorylation of paxillin

Cellular Signalling
Zhi HuangBao-Xue Ge

Abstract

The adaptor protein paxillin plays an important role in cell migration. Although the c-Jun amino-terminal kinase (JNK) phosphorylation of paxillin on Ser 178 has been found to be critical for cell migration, the precise mechanism by which JNK regulates cell migration is still not very clear. Here, the migration of human corneal epithelial (HCE) cells was used to determine which signaling pathways are involved in EGF-induced paxillin phosphorylation. Paxillin was phosphorylated on Tyr 31 and Tyr 118 after induction of migration by EGF in HCE cells. Specific inhibition of JNK activation by inhibitor SP600125 or overexpression of a dominant-negative JNK mutant not only blocked EGF-induced cell migration, but also eliminated tyrosine phosphorylation of paxillin on Tyr 31 and Tyr 118. HCE cells overexpressing paxillin-S178A mutant also exhibited lower mobility, and reduced phosphorylation of Tyr 31 and Tyr 118. However, paxillin-S178A-inhibited cell migration can be rescued by overexpression of paxillin-Y31E/Y118E mutant. Importantly, inhibition of JNK by SP600125 or overexpression of paxillin-S178A mutant prevented the association of FAK with paxillin. Taken together, these results suggest that phosphorylation of paxillin on Ser 17...Continue Reading

References

Jan 25, 1991·Cell·L C CantleyS Soltoff
Jul 1, 1995·Molecular Biology of the Cell·M YebraD A Cheresh
May 1, 1995·Cell Biology International·J BoonstraP van Bergen en Henegouwen
Mar 21, 1998·The Journal of Cell Biology·R L KlemkeD A Cheresh
Aug 7, 1998·Molecular Biology of the Cell·S M ShortR L Juliano
Nov 11, 1999·Oncogene·B E Stronach, N Perrimon
Jul 26, 2000·The Journal of Cell Biology·D K MorrisonV Cleghon
Nov 1, 2000·Cell·J Schlessinger
Jul 11, 2003·Nature·Cai HuangKen Jacobson
Oct 26, 2005·Cell Biochemistry and Biophysics·Rebecca A MacCorkle, Tse-Hua Tan
Oct 29, 2005·Journal of Cell Science·Donna J WebbA Rick Horwitz
Jul 11, 2006·Nature Reviews. Molecular Cell Biology·Ami Citri, Yosef Yarden

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Citations

Jul 31, 2013·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Wei WeiXiaohong Shen
Nov 1, 2011·Cell Communication and Signaling : CCS·Jens RauchWalter Kolch
Mar 29, 2014·Advances in Wound Care·Susan B HopkinsonJonathan C R Jones
Apr 23, 2010·Journal of Cell Science·Ronen Zaidel-Bar, Benjamin Geiger
Oct 26, 2010·Seminars in Cancer Biology·Lihua MinLijian Hui
Aug 7, 2012·European Journal of Immunology·Yu-Chi LeeMing-Zong Lai
Nov 6, 2015·Journal of Cellular Biochemistry·Aisi ZhuHuanxiang Zhang
Jul 4, 2012·Cellular Signalling·Yuki MiyamotoJunji Yamauchi
May 8, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Kazuhiro KimuraKoh-Hei Sonoda
Nov 7, 2013·Cell Adhesion & Migration·Evelyn Png, Louis Tong
Oct 29, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yih-Shou HsiehPei-Ni Chen
Oct 11, 2011·The American Journal of Pathology·Yu QinBob van de Water
Jan 28, 2017·Small GTPases·Neus PedrazaFrancisco Ferrezuelo
Apr 5, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Silvia DragoniPatric Turowski
Oct 9, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Minchul SeoKyoungho Suk
Aug 30, 2018·BioMed Research International·Xingjun WangLei Xue
Apr 15, 2020·International Journal of Molecular Sciences·Takashi SembaNaoto T Ueno
Mar 22, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Panfeng FuViswanathan Natarajan
Nov 12, 2017·Scientific Reports·Ángel Bernabé-GarcíaFrancisco J Nicolás
Aug 7, 2017·Experimental and Therapeutic Medicine·Huaibin LiuChong-Lin Zhang
Feb 20, 2017·Journal of Hematology & Oncology·Ana María López-ColoméEdith López
Feb 13, 2021·International Journal of Molecular Sciences·Korinn N Murphy, Amanda J Brinkworth

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