JNK1 phosphorylation of SCG10 determines microtubule dynamics and axodendritic length.

The Journal of Cell Biology
Tatsiana TararukEleanor T Coffey

Abstract

c-Jun NH(2)-terminal kinases (JNKs) are essential during brain development, when they regulate morphogenic changes involving cell movement and migration. In the adult, JNK determines neuronal cytoarchitecture. To help uncover the molecular effectors for JNKs in these events, we affinity purified JNK-interacting proteins from brain. This revealed that the stathmin family microtubule-destabilizing proteins SCG10, SCLIP, RB3, and RB3' interact tightly with JNK. Furthermore, SCG10 is also phosphorylated by JNK in vivo on sites that regulate its microtubule depolymerizing activity, serines 62 and 73. SCG10-S73 phosphorylation is significantly decreased in JNK1-/- cortex, indicating that JNK1 phosphorylates SCG10 in developing forebrain. JNK phosphorylation of SCG10 determines axodendritic length in cerebrocortical cultures, and JNK site-phosphorylated SCG10 colocalizes with active JNK in embryonic brain regions undergoing neurite elongation and migration. We demonstrate that inhibition of cytoplasmic JNK and expression of SCG10-62A/73A both inhibited fluorescent tubulin recovery after photobleaching. These data suggest that JNK1 is responsible for regulation of SCG10 depolymerizing activity and neurite elongation during brain develo...Continue Reading

References

Jul 1, 1990·The Journal of Cell Biology·S S LimG G Borisy
Mar 28, 1997·The Journal of Biological Chemistry·S B HorwitzU K Schubart
Sep 10, 1998·Biochemical and Biophysical Research Communications·C G ParkerH S Lichenstein
Mar 4, 1999·The EMBO Journal·V AdlerZ Ronai
Apr 2, 1999·The European Journal of Neuroscience·M J Courtney, E T Coffey
Aug 18, 2000·The European Journal of Neuroscience·R LutjensG Grenningloh
Mar 30, 2001·The Journal of Biological Chemistry·E CharbautA Sobel
Sep 20, 2001·Genes & Development·A J WhitmarshR J Davis
Feb 13, 2002·The American Journal of Pathology·Wolfgang LiedtkeUlrich K Schubart
Feb 21, 2002·Molecular Biology of the Cell·Sylvie OzonAndré Sobel
Oct 23, 2002·Journal of Neuroscience Research·Nozomu Mori, Hiroshi Morii
May 21, 2003·Genes & Development·Claire R WestonRoger J Davis
Aug 15, 2003·Molecular and Cellular Biology·Vesa HongistoEleanor T Coffey
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chia-Yi KuanPasko Rakic
Feb 7, 2004·The EMBO Journal·Amos GdalyahuOrly Reiner
Feb 27, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Leejee H SuhDavid W Sretavan
Mar 20, 2004·Science·Philipp NiethammerEric Karsenti
Apr 23, 2004·Trends in Cell Biology·Ying Xia, Michael Karin
Dec 24, 2004·Biochimica Et Biophysica Acta·William H Stoothoff, Gail V W Johnson
Jul 8, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Benny BjörkblomEleanor T Coffey

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Citations

Jul 13, 2013·Neuroscience Bulletin· SaijilafuFeng-Quan Zhou
Feb 23, 2013·Behavioural Brain Research·Kirstin ReineckeThomas Schiffelholz
Sep 24, 2011·Cell Death and Differentiation·G YangM Cynader
Jun 23, 2012·Nature·Matthew J EllisElaine R Mardis
Oct 12, 2010·Nature Neuroscience·Freddy JeanneteauMoses V Chao
Feb 8, 2011·Nature Neuroscience·Nina WesterlundEleanor T Coffey
Jun 12, 2012·Nature Neuroscience·Froylan Calderon de AndaLi-Huei Tsai
Apr 21, 2009·Nature Reviews. Neuroscience·Cecilia Conde, Alfredo Cáceres
Nov 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jung Eun ShinAaron DiAntonio
Sep 18, 2009·The Journal of Biological Chemistry·Vanessa DaireChristian Poüs
Jul 16, 2010·The Journal of Biological Chemistry·Dominic C H NgMarie A Bogoyevitch
Oct 30, 2013·The Journal of Experimental Medicine·Christine D PozniakJoseph W Lewcock
Sep 26, 2013·Brain : a Journal of Neurology·Giovanni NardoCaterina Bendotti
Jan 29, 2011·Human Molecular Genetics·David CheishviliAharon Razin
Nov 26, 2010·Cold Spring Harbor Perspectives in Biology·Erik W DentFrank B Gertler
May 11, 2010·Cold Spring Harbor Perspectives in Biology·F Polleux, William Snider
Feb 18, 2011·Genes & Development·Ping XuRoger J Davis
Apr 29, 2009·Molecular and Cellular Biology·Toru SaigaKeiichi I Nakayama
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Marie A Bogoyevitch, Bostjan Kobe
Jul 13, 2013·Annual Review of Cell and Developmental Biology·Andrew D Chisholm
Apr 13, 2012·Journal of Signal Transduction·Tokiwa YamasakiHiroshi Nishina
Apr 2, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lynn C UsherJohn L Bixby
Oct 22, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ethan R GrafAaron DiAntonio
Aug 2, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Susan KlinedinstCatherine A Collins
Nov 20, 2013·Experimental Neurology·Jung Eun ShinAaron DiAntonio
Jun 28, 2014·Neuroscience Bulletin·Guofa Liu, Trisha Dwyer
Oct 8, 2011·Expert Opinion on Therapeutic Targets·Barbara Belletti, Gustavo Baldassarre
Oct 31, 2012·The Journal of Cell Biology·Bhuvaneish Thangaraj SelvarajMichael Sendtner
Sep 7, 2011·The Journal of Cell Biology·Arundhati Sengupta GhoshJoseph W Lewcock
Feb 24, 2016·Frontiers in Cellular Neuroscience·Chengfu CaiYingzi He
May 23, 2012·Neurobiology of Aging·Chengyu ShengShengdi Chen
Dec 14, 2011·Trends in Neurosciences·Eun-Mi HurFeng-Quan Zhou
Dec 24, 2015·Frontiers in Cellular Neuroscience·Ai-Sze Ching, Azlina Ahmad-Annuar
Feb 25, 2011·Archives of Biochemistry and Biophysics·Vanessa Daire, Christian Poüs
Jul 17, 2010·Cellular Signalling·Wiebke HaeusgenVicki Waetzig
Oct 8, 2009·Neuroscience Research·Kaoru EtoKazunori Nakajima
Aug 8, 2009·Molecular and Cellular Neurosciences·Fabienne E Poulain, André Sobel

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Methods Mentioned

BETA
pull down
immunoprecipitation
coimmunoprecipitation
transfection
PCR
affinity purification
FCS

Software Mentioned

Wasabi
EL
MetaMorph
KG

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