JNK/ATF2 pathway is involved in iodinated contrast media-induced apoptosis

American Journal of Nephrology
Hsiang-Chun LeeJan-Gowth Chang

Abstract

Notably, activating transcriptional factor 2 (ATF2), a histone-modification gene, is involved in oxidative stress-induced apoptosis. The aim of this study was to clarify the role of ATF2 in contrast media-induced nephropathy. Human embryonic kidney 293T cells were treated with four different contrast media:ionic high-osmolar diatrizoate; ionic low-osmolar iothalamate; non-ionic low-osmolar iohexol, and non-ionic iso-osmolar iodixanol. The mRNA expression of ATF2 was determined by real-time PCR. Short interfering RNA was used to knock down ATF2 mRNA expression. Phosphorylation of ATF2 was measured by Western blotting. Wistar rats were administered either diatrizoate or a normal saline injection. Apoptosis in kidney tubular cells was determined by the presence of positive TUNEL stain. Diatrizoate, iodixanol and iothalamate, but not iohexol, induced the expression of ATF2 mRNA and phosphorylation of ATF2 in 293T cells in a time-dependent manner. More apoptotic cells were in diatrizoate-treated kidney cells than in the saline injection group (p < 0.00001). Cell death was significantly increased by knockdown ATF2 expression in the presence of diatrizoate, indicating a protective role of ATF2 in contrast media-induced apoptosis. Diff...Continue Reading

Citations

Nov 3, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Michele AndreucciAshour Michael
Feb 26, 2011·Clinical and Experimental Pharmacology & Physiology·Mauricio M Sendeski
Apr 20, 2014·BioMed Research International·Ashour MichaelMichele Andreucci
Jul 26, 2011·International Journal of Cardiology·Philip Ching Yat WongAidong Zhang
Sep 19, 2012·Vascular Pharmacology·Nicoletta RondaEnrico Fiaccadori
Jan 21, 2015·Chemico-biological Interactions·Michele AndreucciAshour Michael
Jul 6, 2016·Journal of Experimental & Clinical Cancer Research : CR·Deng-Shuang WuLin-Hui Wang
Nov 14, 2012·Circulation·Cristina QuintavalleGerolama Condorelli
Mar 27, 2012·Pacing and Clinical Electrophysiology : PACE·Kun-Tai LeeWen-Ter Lai
Oct 19, 2017·Journal of Cellular Physiology·Michele AndreucciAshour Michael
Oct 22, 2020·Journal of Translational Medicine·Prit KusirisinNipon Chattipakorn
Sep 6, 2020·Life Sciences·Fangfei ZhangFeng Wang
Oct 21, 2017·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Georges VassauxBéatrice Cambien
Jul 27, 2021·Disease-a-month : DM·Gates B ColbertEdgar Lerma

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