Joining mutants of RAG1 and RAG2 that demonstrate impaired interactions with the coding-end DNA.

The Journal of Biological Chemistry
Fumikiyo NagawaHitoshi Sakano

Abstract

In V(D)J joining of antigen receptor genes, two recombination signal sequences (RSSs), 12- and 23-RSSs, form a complex with the protein products of recombination activating genes, RAG1 and RAG2. DNaseI footprinting demonstrates that the interaction of RAG proteins with substrate RSS DNA is not just limited to the signal region but involves the coding sequence as well. Joining mutants of RAG1 and RAG2 demonstrate impaired interactions with the coding region in both pre- and postcleavage type complexes. A possible role of this RAG coding region interaction is discussed in the context of V(D)J recombination.

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Citations

Aug 26, 2004·Nucleic Acids Research·Sadiqur R TalukderYoshiko Akamatsu
Jan 8, 2013·Nucleic Acids Research·Mihai CiubotaruDavid G Schatz
Jul 20, 2007·Molecular and Cellular Biology·Anna H Drejer-TeelDavid G Schatz
Oct 7, 2011·The Journal of Clinical Endocrinology and Metabolism·Sven GläskerEdward H Oldfield
Aug 23, 2011·Annual Review of Genetics·David G Schatz, Patrick C Swanson
Dec 17, 2009·Seminars in Immunology·Sebastian D Fugmann
Feb 7, 2006·Current Opinion in Immunology·Salvatore SpicugliaPierre Ferrier
Dec 20, 2007·The Journal of Biological Chemistry·Tadashi NishiharaHitoshi Sakano

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