Joint Genomic and Proteomic Analysis Identifies Meta-Trait Characteristics of Virulent and Non-virulent Staphylococcus aureus Strains

Frontiers in Cellular and Infection Microbiology
Emilia A BonarBenedykt Wladyka

Abstract

Staphylococcus aureus is an opportunistic pathogen of humans and warm-blooded animals and presents a growing threat in terms of multi-drug resistance. Despite numerous studies, the basis of staphylococcal virulence and switching between commensal and pathogenic phenotypes is not fully understood. Using genomics, we show here that S. aureus strains exhibiting virulent (VIR) and non-virulent (NVIR) phenotypes in a chicken embryo infection model genetically fall into two separate groups, with the VIR group being much more cohesive than the NVIR group. Significantly, the genes encoding known staphylococcal virulence factors, such as clumping factors, are either found in different allelic variants in the genomes of NVIR strains (compared to VIR strains) or are inactive pseudogenes. Moreover, the pyruvate carboxylase and gamma-aminobutyrate permease genes, which were previously linked with virulence, are pseudogenized in NVIR strain ch22. Further, we use comprehensive proteomics tools to characterize strains that show opposing phenotypes in a chicken embryo virulence model. VIR strain CH21 had an elevated level of diapolycopene oxygenase involved in staphyloxanthin production (protection against free radicals) and expressed a higher ...Continue Reading

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Citations

Dec 5, 2019·Scientific Reports·Isana Maria de Souza Feitosa LimaLuís Cláudio Nascimento da Silva
Feb 12, 2021·Clinical Microbiology Reviews·Soyoun Park, Jennifer Ronholm
May 2, 2021·International Journal of Biological Macromolecules·Emilia BonarBenedykt Wladyka

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Datasets Mentioned

BETA
CP001781.1
CP017809
CP014420.1
BA000018.3

Methods Mentioned

BETA
PCR
electrophoresis
gene knock-outs

Software Mentioned

BLAST
CSI phylogeny
RAxML
BLAST +
MEGA6
muscle
Mascot
Circos
CLC Genomics Workbench
Clustal Omega

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