Joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies

Journal of Biomolecular NMR
Mateusz UrbańczykKrzysztof Kazimierczuk

Abstract

NMR relaxometry plays crucial role in studies of protein dynamics. The measurement of longitudinal and transverse relaxation rates of [Formula: see text]N is the main source of information on backbone motions. However, even the most basic approach exploiting a series of [Formula: see text]N HSQC spectra can require several hours of measurement time. Standard non-uniform sampling (NUS), i.e. random under-sampling of indirect time domain, typically cannot reduce this by more than 2-4[Formula: see text] due to relatively low "compressibility" of these spectra. In this paper we propose an extension of NUS to relaxation delays. The two-dimensional space of [Formula: see text]/[Formula: see text] is sampled in a way similar to NUS of [Formula: see text]/[Formula: see text] domain in 3D spectra. The signal is also processed in a way similar to that known from 3D NUS spectra i.e. using one of the most popular compressed sensing algorithms, iterative soft thresholding. The 2D Fourier transform matrix is replaced with mixed inverse Laplace-Fourier transform matrix. The peak positions in resulting 3D spectrum are characterized by two frequency coordinates and relaxation rate and thus no additional fitting of exponential curves is required...Continue Reading

References

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Nov 12, 2016·Journal of Biomolecular NMR·Alexandra ShchukinaKrzysztof Kazimierczuk

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Citations

Jul 8, 2019·Journal of Biomolecular NMR·Jared RovnySean E Barrett
Jun 18, 2017·Journal of Biomolecular NMR·Martin Billeter
Oct 10, 2020·The Journal of Chemical Physics·Deepansh J Srivastava, Philip J Grandinetti
Jun 20, 2020·Analytical Chemistry·Mateusz UrbańczykVille-Veikko Telkki

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Methods Mentioned

BETA
NMR

Software Mentioned

coMDD
ITAMeD
MATLAB

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