Joint regression-classification deep learning framework for analyzing fluorescence lifetime images using NADH and FAD.

Biomedical Optics Express
Lopamudra MukherjeeKevin W Eliceiri

Abstract

In this paper, we develop a deep neural network based joint classification-regression approach to identify microglia, a resident central nervous system macrophage, in the brain using fluorescence lifetime imaging microscopy (FLIM) data. Microglia are responsible for several key aspects of brain development and neurodegenerative diseases. Accurate detection of microglia is key to understanding their role and function in the CNS, and has been studied extensively in recent years. In this paper, we propose a joint classification-regression scheme that can incorporate fluorescence lifetime data from two different autofluorescent metabolic co-enzymes, FAD and NADH, in the same model. This approach not only represents the lifetime data more accurately but also provides the classification engine a more diverse data source. Furthermore, the two components of model can be trained jointly which combines the strengths of the regression and classification methods. We demonstrate the efficacy of our method using datasets generated using mouse brain tissue which show that our joint learning model outperforms results on the coenzymes taken independently, providing an efficient way to classify microglia from other cells.

References

Apr 6, 1990·Science·W DenkW W Webb
Mar 29, 2000·Biophysical Journal·P J VerveerP I Bastiaens
Dec 17, 2003·Microscopy Research and Technique·Ye Chen, Ammasi Periasamy
Jul 15, 2004·Journal of Biomedical Optics·Javier A JoLaura Marcu
Jun 17, 2005·Journal of Neuroscience Research·Jyoti J WattersBehnam Badie
Sep 23, 2006·BioTechniques·Long YanKevin W Eliceiri
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Gwenn A Garden, Thomas Möller
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Melissa C SkalaNirmala Ramanujam
Nov 26, 2008·Journal of Leukocyte Biology·Bart R TambuyzerEtienne J Nouwen
Jul 29, 2009·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Adrian Barbu
Mar 30, 2011·Glia·Nikki A CharlesHelmut Kettenmann
Feb 16, 2013·Biomedical Optics Express·Mohammad A YaseenDavid A Boas
Aug 24, 2013·IEEE Transactions on Pattern Analysis and Machine Intelligence·Justin Domke
Aug 13, 2015·BMC Medical Imaging·Abdel Aziz Taha, Allan Hanbury
Oct 9, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jenu V Chacko, Kevin W Eliceiri
Nov 14, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jason T SmithXavier Intes
Jan 24, 2020·Methods and Applications in Fluorescence·Ruofan CaoAmmasi Periasamy
Oct 6, 2020·Frontiers in Neuroscience·Md Abdul Kader SagarKevin W Eliceiri

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