Joint use of small-angle X-ray and neutron scattering to study biological macromolecules in solution.

European Biophysics Journal : EBJ
Maxim V Petoukhov, Dmitri I Svergun

Abstract

Novel techniques for simultaneous analysis of X-ray and neutron scattering patterns from macromolecular complexes in solution are presented. They include ab initio shape and internal structure determination of multicomponent particles and more detailed rigid body modeling of complexes using high resolution structures of subunits. The methods fit simultaneously X-ray and neutron scattering curves including contrast variation data sets from selectively deuterated complexes. Biochemically sound interconnected models without steric clashes between the components displaying a pre-defined symmetry are generated. For rigid body modeling, distance restraints between specified residues/nucleotides or their ranges are taken into account. The efficiency of the methods is demonstrated in model examples, and potential sources of ambiguity are discussed.

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Citations

Feb 4, 2011·European Biophysics Journal : EBJ·Julia PreuThomas Gutberlet
Jun 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Michelle P ChristieJennifer L Martin
Apr 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jean-Marie BourhisDavid J S Hulmes
Dec 12, 2012·Annual Review of Physical Chemistry·Clement E Blanchet, Dmitri I Svergun
Mar 8, 2016·Archives of Biochemistry and Biophysics·Bente Vestergaard
Jul 14, 2009·Journal of Molecular Biology·Jianquan LiZimei Bu
Mar 14, 2007·Journal of Molecular Biology·Andrew E WhittenDavid B Langley
Sep 20, 2008·Current Opinion in Structural Biology·H Tsuruta, T C Irving
Jun 19, 2010·Journal of Structural Biology·Haydyn D T Mertens, Dmitri I Svergun
Dec 17, 2008·Structure·Qi WangHolger Sondermann
Jan 25, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christian E H SchmelzerLaurent Muller
Apr 6, 2019·Acta Crystallographica. Section D, Structural Biology·Line A RybergPernille Harris
Jan 1, 2014·Frontiers in Molecular Biosciences·Maxim V PetoukhovDmitri I Svergun
Aug 21, 2020·The Journal of Physical Chemistry. B·Maksym GolubJörg Pieper
Jul 2, 2021·Biophysical Journal·Mateusz WilamowskiGreg L Hura

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