Jolkinolide B induces neuroendocrine differentiation of human prostate LNCaP cancer cell line

Biochemical Pharmacology
W K LiuChun-Tao Che

Abstract

Euphorbia fischeriana is a Chinese herbal medicine which has been reported to possess chemotherapeutic effects, yet the underlying mechanism is unclear. In order to understand its possible anti-tumor property, we have isolated a number of chemical compounds from the roots of this plant [Phytochemistry 52 (1999) 117] and studied their in vitro effects by using human prostate LNCaP cancer cell line. Among the six compounds tested, jolkinolide B exhibited the most potent anti-proliferative activity (IC(50)=12.5 microg/mL=40 microM) and it inhibited DNA synthesis by down-regulating bromodeoxyuridine (BrdU) incorporation in LNCaP cells in a dose-dependent manner. Jolkinolide B, at concentrations up to 25 microg/mL, induced G1 arrest and neuroendocrine differentiation of LNCaP cells. Immunoblotting analysis confirmed the increased expression of neuroendocrine markers, keratin 8/18 (K8/18) and neuron specific enolase (NSE), in these cells. Apoptotic bodies and DNA fragmentation were observed by fluorescence microscopy and flow cytometry when the cells were exposed to a concentration higher than 25 microg/mL jolkinolide B. Taken all data together, jolkinolide B seems to play a role in the regulation of proliferation, differentiation, a...Continue Reading

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Citations

Feb 16, 2006·Journal of Cancer Research and Clinical Oncology·Zahra AmirghofranKatayoun Javidnia
Mar 18, 2004·Biochimica Et Biophysica Acta·J C K HoW K Liu
Mar 24, 2012·Journal of Natural Products·Wing Keung LiuChun-Tao Che
Jan 16, 2007·Canadian Journal of Physiology and Pharmacology·Huiying Luo, Aiqin Wang
Sep 29, 2015·Journal of Natural Medicines·Xinzhu KuangKazuo Koike
Mar 6, 2012·Phytotherapy Research : PTR·Shuli ManChangxiao Liu
Sep 15, 2004·European Journal of Pharmacology·Wing-Keung LiuChun-Tao Che
Jan 24, 2012·Journal of Ethnopharmacology·Shengpeng WangYitao Wang
Feb 5, 2014·Biochemical and Biophysical Research Communications·Xiaojun MaDewei Zhao
Mar 1, 2006·Current Therapeutic Research, Clinical and Experimental·Xiaohua NieWenyi Tao
Dec 4, 2016·Bioorganic & Medicinal Chemistry Letters·Qun ShiDali Meng
Feb 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Baiyu JianJicheng Liu
Mar 25, 2010·Immunopharmacology and Immunotoxicology·Zahra AmirghofranFatemeh Kalalinia
Sep 6, 2005·Molecular Pharmacology·Qinghai TianMing-Wei Wang
Jul 19, 2011·Chemistry & Biodiversity·Yong-Xu Sun, Ji-Cheng Liu

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