Bone is a complex tissue with a variety of functions, such as providing mechanical stability for locomotion, protection of the inner organs, mineral homeostasis and haematopoiesis. To fulfil these diverse roles in the human body, bone consists of a multitude of different cells and an extracellular matrix that is mechanically stable, yet flexible at the same time. Unlike most tissues, bone is under constant renewal facilitated by a coordinated interaction of bone-forming and bone-resorbing cells. It is thus challenging to recreate bone in its complexity in vitro and most current models rather focus on certain aspects of bone biology that are of relevance for the research question addressed. In addition, animal models are still regarded as the gold-standard in the context of bone biology and pathology, especially for the development of novel treatment strategies. However, species-specific differences impede the translation of findings from animal models to humans. The current review summarizes and discusses the latest developments in bone tissue engineering and organoid culture including suitable cell sources, extracellular matrices and microfluidic bioreactor systems. With available technology in mind, a best possible bone model...Continue Reading
The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells
Species differences in growth requirements for bone marrow stromal fibroblast colony formation In vitro
Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow.
Modulation of marrow stromal osteoblast adhesion on biomimetic oligo[poly(ethylene glycol) fumarate] hydrogels modified with Arg-Gly-Asp peptides and a poly(ethyleneglycol) spacer
Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner
Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types
Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity
Development of a mechanical testing and loading system for trabecular bone studies for long term culture
Comparison of microcomputed tomographic and microradiographic measurements of cortical bone porosity
Modulation of in vitro angiogenesis in a three-dimensional spheroidal coculture model for bone tissue engineering
The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells
Three-dimensional perfusion culture of human bone marrow cells and generation of osteoinductive grafts
Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals
Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue
Simulation of cell differentiation in fracture healing: mechanically loaded composite scaffolds in a novel bioreactor system
Development of magnetic particle techniques for long-term culture of bone cells with intermittent mechanical activation
Principles and design of a novel magnetic force mechanical conditioning bioreactor for tissue engineering, stem cell conditioning, and dynamic in vitro screening
Bone regeneration using hyaluronic acid-based hydrogel with bone morphogenic protein-2 and human mesenchymal stem cells
Three-dimensional perfusion culture of human adipose tissue-derived endothelial and osteoblastic progenitors generates osteogenic constructs with intrinsic vascularization capacity
A review of materials, fabrication methods, and strategies used to enhance bone regeneration in engineered bone tissues
Femtosecond Laser Fabrication of Engineered Functional Surfaces Based on Biodegradable Polymer and Biopolymer/Ceramic Composite Thin Films
Bone Tissue Regeneration in the Oral and Maxillofacial Region: A Review on the Application of Stem Cells and New Strategies to Improve Vascularization
Finite Element Analysis of Orthopedic Hip Implant with Functionally Graded Bioinspired Lattice Structures
Design and Evaluation of an Osteogenesis-on-a-Chip Microfluidic Device Incorporating 3D Cell Culture
Functional Scaffold-Free Bone Equivalents Induce Osteogenic and Angiogenic Processes in a Human In Vitro Fracture Hematoma Model.
Trabecular bone organoids: a micron-scale 'humanised' prototype designed to study the effects of microgravity and degeneration.
Bio-inspired multifunctional collagen/electrospun bioactive glass membranes for bone tissue engineering applications.
Co-culture systems of osteoblasts and osteoclasts: Simulating in vitro bone remodeling in regenerative approaches.
Overcoming the Dependence on Animal Models for Osteoarthritis Therapeutics - The Promises and Prospects of In Vitro Models.
Biomimetic Hierarchical Structuring of PLA by Ultra-Short Laser Pulses for Processing of Tissue Engineered Matrices: Study of Cellular and Antibacterial Behavior.
3D Printing and Bioprinting to Model Bone Cancer: The Role of Materials and Nanoscale Cues in Directing Cell Behavior.
Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.