JTE-522-induced apoptosis in human gastric adenocarcinoma [correction of adenocarcinoma] cell line AGS cells by caspase activation accompanying cytochrome C release, membrane translocation of Bax and loss of mitochondrial membrane potential

World Journal of Gastroenterology : WJG
Hong-Liang LiCun-Chuan Wang

Abstract

To investigate the role of the mitochondrial pathway in JTE-522-induced apoptosis and to investigate the relationship between cytochrome C release, caspase activity and loss of mitochondrial membrane potential (Deltapsim). Cell culture, cell counting, ELISA assay, TUNEL, flow cytometry, Western blot and fluorometric assay were employed to investigate the effect of JTE-522 on cell proliferation and apoptosis in AGS cells and related molecular mechanism. JTE-522 inhibited the growth of AGS cells and induced the apoptosis. Caspases 8 and 9 were activated during apoptosis as judged by the appearance of cleavage products from procaspase and the caspase activities to cleave specific fluorogenic substrates. To elucidate whether the activation of caspases 8 and 9 was required for the apoptosis induction, we examined the effect of caspase-specific inhibitors on apoptosis. The results showed that caspase inhibitors significantly inhibited the apoptosis induced by JTE-522. In addition, the membrane translocation of Bax and cytosolic release of cytochrome C accompanying with the decrease of the uptake of Rhodamin 123, were detected at an early stage of apoptosis. Furthermore, Bax translocation, cytochrome C release, and caspase 9 activatio...Continue Reading

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Citations

Apr 8, 2003·World Journal of Gastroenterology : WJG·Jun Yan, Yong-Hua Xu
May 30, 2003·Biology of Reproduction·Nicoletta Di SimoneAlessandro Caruso
Apr 30, 2003·World Journal of Gastroenterology : WJG·Xiao-Hong LiXian-Da Ren
Jun 12, 2003·World Journal of Gastroenterology : WJG·Ming-Yi LiXiao-Yu Tan
Apr 11, 2009·World Journal of Gastroenterology : WJG·Yu YaoTu-Sheng Song

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