Jug r 2-reactive CD4(+) T cells have a dominant immune role in walnut allergy

The Journal of Allergy and Clinical Immunology
Luis Diego ArchilaWilliam W Kwok

Abstract

Allergic reactions to walnut can be life-threatening. Although IgE epitopes of walnut have been studied, CD4(+) T cell-specific epitopes for walnut remain uncharacterized. In particular, the relationship of both phenotype and frequency of walnut-specific T cells to the disease have not been examined. We sought to provide a thorough phenotypic analysis for walnut-reactive T cells in allergic and nonallergic subjects, particularly the relationship of phenotypes and frequencies of walnut-specific T cells with the disease. The CD154 upregulation assay was used to examine CD4(+) T-cell reactivity toward the walnut allergens Jug r 1, Jug r 2, and Jug r 3. A tetramer-guided epitope mapping approach was used to identify HLA-restricted CD4(+) T-cell epitopes in Jug r 2. Direct ex vivo staining with peptide-major histocompatibility complex class II tetramers enabled comparison of the frequency and phenotype of Jug r 2-specific CD4(+) T cells between allergic and nonallergic subjects. Jug r 2-specific T-cell clones were also generated, and mRNA transcription factor levels were assessed by using quantitative RT-PCR. Intracellular cytokine staining assays were performed for further phenotypic analyses. Jug r 2 was identified as the major al...Continue Reading

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Citations

Mar 10, 2016·The Journal of Allergy and Clinical Immunology·Manish RameshHugh A Sampson
Dec 27, 2015·Allergy·Ulrich M ZisslerCarsten B Schmidt-Weber
Jun 18, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·William W Kwok
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Apr 30, 2016·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L D ArchilaW W Kwok
Mar 28, 2017·The Journal of Allergy and Clinical Immunology·L Diego ArchilaWilliam W Kwok
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Apr 28, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J M SmeekensM Kulis
Jul 11, 2018·Frontiers in Immunology·Amedee RenandWilliam W Kwok
Jun 3, 2018·Mucosal Immunology·Petra Bacher, Alexander Scheffold
Dec 12, 2018·The Journal of Allergy and Clinical Immunology·Petra Bacher, Alexander Scheffold

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Methods Mentioned

BETA
PCR
flow-cytometry

Software Mentioned

FlowJo

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