Juglone suppresses epithelial-mesenchymal transition in prostate cancer cells via the protein kinase B/glycogen synthase kinase-3β/Snail signaling pathway

Oncology Letters
Fang FangLiguo Wang

Abstract

Epithelial-mesenchymal transition (EMT) serves an important role in the metastasis of prostate cancer. Juglone is a natural compound isolated from plants that is reported to possess potent cytotoxic properties. However, there are no studies on the anti-EMT effect of juglone in prostate cancer, or its potential underlying mechanisms of action. In the present study, the effect of juglone on the EMT of prostate cancer cells was investigated. Transwell assays were used to demonstrate that juglone inhibits the migration and invasion of the prostate cancer (PC) LNCaP and LNCaP-AI cell lines. Results from western blot analysis demonstrated that juglone increases the expression of the epithelial marker E-cadherin while decreasing the expression of mesenchymal markers (N-cadherin and Vimentin) in a dose-dependent manner. The data from the present study also revealed that juglone downregulates the expression of Snail, a repressor of E-cadherin and an inducer of EMT. Furthermore, juglone prevented inactivation of glycogen synthase kinase-3β (GSK-3β), an endogenous inhibitor of Snail in a dose-dependent manner. Lithium chloride (LiCl), a GSK-3β inhibitor, prevented juglone-mediated downregulation of Snail expression and upregulation of E-c...Continue Reading

References

Apr 16, 2004·The New England Journal of Medicine·G David Roodman
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Jan 29, 2010·Cancer·Toni IbrahimDino Amadori
Jan 5, 2011·International Review of Cell and Molecular Biology·Douglas R Hurst, Danny R Welch
Jan 24, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hua Li XuDa Yuan Sui
Nov 28, 2012·Canadian Journal of Physiology and Pharmacology·Wei ZhangYing Jin
Jan 15, 2014·Cancer Metastasis Reviews·Rhonda L BittingAndrew J Armstrong
Mar 25, 2014·Molecular Cancer Therapeutics·Mercedes Marín-AguileraBegoña Mellado
Aug 3, 2014·Journal of Experimental & Clinical Cancer Research : CR·Samantha Kaufhold, Benjamin Bonavida
May 30, 2015·Molecular Cancer Research : MCR·Alvaro Gutierrez-UzquizaMarcelo G Kazanietz
Aug 21, 2015·Connective Tissue Research·Jacqueline Banyard, Diane R Bielenberg

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