Jump around: transposons in and out of the laboratory

F1000Research
Anuj Kumar

Abstract

Since Barbara McClintock's groundbreaking discovery of mobile DNA sequences some 70 years ago, transposable elements have come to be recognized as important mutagenic agents impacting genome composition, genome evolution, and human health. Transposable elements are a major constituent of prokaryotic and eukaryotic genomes, and the transposition mechanisms enabling transposon proliferation over evolutionary time remain engaging topics for study, suggesting complex interactions with the host, both antagonistic and mutualistic. The impact of transposition is profound, as over 100 human heritable diseases have been attributed to transposon insertions. Transposition can be highly mutagenic, perturbing genome integrity and gene expression in a wide range of organisms. This mutagenic potential has been exploited in the laboratory, where transposons have long been utilized for phenotypic screening and the generation of defined mutant libraries. More recently, barcoding applications and methods for RNA-directed transposition are being used towards new phenotypic screens and studies relevant for gene therapy. Thus, transposable elements are significant in affecting biology both in vivo and in the laboratory, and this review will survey a...Continue Reading

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Citations

Jun 18, 2021·Stem Cell Research & Therapy·Gabrielle M RobbinsBranden S Moriarity
Jul 3, 2021·Life·Agrita PuzukaJuris Erenpreiss

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Methods Mentioned

BETA
X-ray
chemical treatments
amplicon sequencing
transgenic
biopsy
xenograft

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