Junctate is a Ca2+-sensing structural component of Orai1 and stromal interaction molecule 1 (STIM1).

Proceedings of the National Academy of Sciences of the United States of America
Sonal SrikanthYousang Gwack

Abstract

Orai1 and stromal interaction molecule (STIM)1 are critical components of Ca(2+) release-activated Ca(2+) (CRAC) channels. Orai1 is a pore subunit of CRAC channels, and STIM1 acts as an endoplasmic reticulum (ER) Ca(2+) sensor that detects store depletion. Upon store depletion after T-cell receptor stimulation, STIM1 translocates and coclusters with Orai1 at sites of close apposition of the plasma membrane (PM) and the ER membrane. However, the molecular components of these ER-PM junctions remain poorly understood. Using affinity protein purification, we uncovered junctate as an interacting partner of Orai1-STIM1 complex. Furthermore, we identified a Ca(2+)-binding EF-hand motif in the ER-luminal region of junctate. Mutation of this EF-hand domain of junctate impaired its Ca(2+) binding and resulted in partial activation of CRAC channels and clustering of STIM1 independently of store depletion. In addition to the known mechanisms of STIM1 clustering (i.e., phosphoinositide and Orai1 binding), our study identifies an alternate mechanism to recruit STIM1 into the ER-PM junctions via binding to junctate. We propose that junctate, a Ca(2+)-sensing ER protein, is a structural component of the ER-PM junctions where Orai1 and STIM1 cl...Continue Reading

References

Aug 19, 2000·Molecular Cell·H TakeshimaK Kangawa
Dec 12, 2001·Biochemical and Biophysical Research Communications·C S HongDo H Kim
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
Sep 24, 1955·Nature·G N RAMACHANDRAN, G KARTHA
Jul 12, 2005·Current Biology : CB·Jen LiouTobias Meyer
Oct 19, 2006·Biochemical and Biophysical Research Communications·Pingyong XuTao Xu
Feb 13, 2007·The Journal of Biological Chemistry·Yousang GwackAnjana Rao
Apr 5, 2007·Nature Protocols·Holger LorenzJennifer Lippincott-Schwartz
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jen LiouTobias Meyer
Aug 19, 2007·The Journal of Biological Chemistry·Zhengzheng LiTao Xu
Aug 21, 2007·Biochemical and Biophysical Research Communications·Chang-Soo HongDo Han Kim
Feb 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Maria I LioudynoMichael D Cahalan
Jun 2, 2009·The Journal of Biological Chemistry·Marek K KorzeniowskiTamas Balla
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Franklin M MullinsRichard S Lewis
Oct 22, 2009·The Biochemical Journal·Ciara M WalshRobert D Burgoyne
Nov 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Lelio OrciPierre Cosson
Mar 24, 2010·Annual Review of Immunology·Patrick G HoganAnjana Rao
Nov 11, 2010·Journal of Cell Science·Susan TrevesFancesco Zorzato
May 10, 2011·Annual Review of Biochemistry·Silvia Carrasco, Tobias Meyer
Aug 30, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kyun-Do KimYousang Gwack

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Citations

Jul 18, 2012·Cellular and Molecular Life Sciences : CMLS·Martin MuikChristoph Romanin
Mar 14, 2013·Molecules and Cells·Sonal Srikanth, Yousang Gwack
Jan 12, 2013·Molecules and Cells·Jeong-Eun LeeJoong-Kook Choi
Aug 24, 2012·Nature Reviews. Molecular Cell Biology·Jonathan SoboloffDonald L Gill
Jan 26, 2013·The Journal of Clinical Investigation·Rajesh Kumar GandhirajanMuniswamy Madesh
Oct 15, 2013·Trends in Cell Biology·Noa Beatriz Martín-CófrecesFrancisco Sánchez-Madrid
Oct 12, 2012·Cellular and Molecular Life Sciences : CMLS·Patrick J ShawStefan Feske
Oct 7, 2014·Journal of Molecular Biology·Ann Hye-Ryong ShimMurali Prakriya
Jan 28, 2016·Science Signaling·Isabella DerlerChristoph Romanin
Mar 26, 2013·Current Opinion in Cell Biology·Christopher J StefanScott D Emr
Mar 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jin Seok WooYousang Gwack
Mar 1, 2016·Biochimica Et Biophysica Acta·Chi-Lun Chang, Jen Liou
Apr 14, 2016·The Journal of Cell Biology·Shyam SrivatsRuth D Murrell-Lagnado
May 27, 2015·The Journal of General Physiology·Eduardo RíosSheila Riazi
Nov 26, 2014·Cellular and Molecular Life Sciences : CMLS·Marion Weber-BoyvatVesa M Olkkonen
Jan 1, 2014·The International Journal of Biochemistry & Cell Biology·Robyn T RebbeckAngela F Dulhunty
Jun 19, 2013·Biochimica Et Biophysica Acta·Andy K M Lam, Antony Galione
Apr 2, 2014·Pathogens and Disease·Felicia D EmeryMark A Miller
May 27, 2016·Nature Communications·Bipan Kumar DebGaiti Hasan
Jan 29, 2017·The Journal of Physiology·Malika FaouziReinhold Penner
Apr 23, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Paula Nunes-Hasler, Nicolas Demaurex
May 31, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Chi-Lun ChangJen Liou
Sep 9, 2018·American Journal of Medical Genetics. Part a·Hugo H Abarca BarrigaRaoul C Hennekam
Oct 17, 2013·Journal of Muscle Research and Cell Motility·Pura BolañosCarlo Caputo
Mar 29, 2014·Pharmacological Reviews·Marie BillaudBrant E Isakson
Jan 31, 2016·American Journal of Physiology. Cell Physiology·Isabella DerlerChristoph Romanin
Oct 2, 2019·Cold Spring Harbor Perspectives in Biology·Richard S Lewis
Mar 17, 2017·Annual Review of Biochemistry·Yasunori Saheki, Pietro De Camilli
Jul 31, 2019·Cold Spring Harbor Perspectives in Biology·Wen-An WangMarek Michalak
Sep 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·Derayvia GrimesRegina A Clemens
Dec 7, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kyun-Do KimYousang Gwack
Mar 25, 2019·European Biophysics Journal : EBJ·Adéla KrizovaIsabella Derler
Jan 1, 2020·International Journal of Molecular Sciences·Lucile NoyerDimitra Gkika
Dec 27, 2016·Frontiers in Cell and Developmental Biology·Bipan K Deb, Gaiti Hasan
Jan 25, 2018·Experimental Biology and Medicine·Heather A Nelson, Michael W Roe
Jun 11, 2017·The Journal of Cell Biology·Yu-Ju ChenJen Liou
Sep 25, 2015·Physiological Reviews·Murali Prakriya, Richard S Lewis
Aug 5, 2020·The Journal of Clinical Investigation·Julia VolzBernhard Nieswandt

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