Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction

Nature Communications
Ilkka PaateroHeinz-Georg Belting

Abstract

Angiogenesis and vascular remodeling are driven by extensive endothelial cell movements. Here, we present in vivo evidence that endothelial cell movements are associated with oscillating lamellipodia-like structures, which emerge from cell junctions in the direction of cell movements. High-resolution time-lapse imaging of these junction-based lamellipodia (JBL) shows dynamic and distinct deployment of junctional proteins, such as F-actin, VE-cadherin and ZO1, during JBL oscillations. Upon initiation, F-actin and VE-cadherin are broadly distributed within JBL, whereas ZO1 remains at cell junctions. Subsequently, a new junction is formed at the front of the JBL, which then merges with the proximal junction. Rac1 inhibition interferes with JBL oscillations and disrupts cell elongation-similar to a truncation in ve-cadherin preventing VE-cad/F-actin interaction. Taken together, our observations suggest an oscillating ratchet-like mechanism, which is used by endothelial cells to move over each other and thus provides the physical means for cell rearrangements.

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Citations

Feb 29, 2020·Current Opinion in Hematology·Florian AlonsoElisabeth Génot
Jul 24, 2020·Angiogenesis·Danielle B BuglakVictoria L Bautch
Dec 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·John Xiao He LiWilliam M Brieher
Aug 28, 2020·Frontiers in Cell and Developmental Biology·Ann-Cathrin WernerEloi Montanez
Jan 5, 2019·Journal of Cell Science·Jiahui Cao, Hans Schnittler
Aug 15, 2020·Frontiers in Physiology·Kazuhide S Okuda, Benjamin M Hogan
Dec 6, 2018·Biochemical Society Transactions·Lilian Schimmel, Emma Gordon
Jan 30, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Zhuoyan LiSun Chen
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Qing Zhang
Dec 12, 2020·Cells·Marie-José BoueidMarcel Tawk
Jun 23, 2021·Seminars in Cell & Developmental Biology·Li-Kun Phng, Heinz-Georg Belting
Aug 13, 2021·Development·Minkyoung LeeHeinz-Georg Belting

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genotyping

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SPSS
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