Juvenile hormone diol kinase. I. Purification, characterization, and substrate specificity of juvenile hormone-selective diol kinase from Manduca sexta

The Journal of Biological Chemistry
Robert A MaxwellD A Schooley

Abstract

Manduca sexta juvenile hormone diol kinase (JHDK) catalyzes the conversion of juvenile hormone (JH) diol to JH diol phosphate. JHDK may be the first example of a phosphotransferase directly involved in the catabolism and inactivation of a lipid-soluble hormone. JHDK is an enzyme crucial for secondary metabolism of JH and possesses high specificity and catalytic efficiency for JH diol. In this study, the purification and characterization of native JHDK are described; its enzymatic properties are examined; and its role in cellular JH metabolism is explored. Using a variety of potential substrates, we show that JHDK has a preference for ATP, but will catalyze the formation of JH diol phosphate with GTP as the phosphate donor. JHDK has a nanomolar K(m) for JH I diol and a low micromolar value for MgATP. JH II and III diols also serve as phosphate acceptors with low micromolar K(m), whereas other diol derivatives of terpenoid esters structurally similar to JH metabolites are not phosphorylated. The reaction proceeds via a sequential Bi Bi mechanism. JHDK is active as a homodimer with a subunit molecular mass of 20 kDa. JHDK binds 5'-p-fluorosulfonylbenzoyladenosine and is inhibited by micromolar levels of Ca2+.

References

Jan 1, 1978·The Biochemical Journal·L G Dann, H G Britton
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Sep 15, 1974·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Slade, C F Wilkinson
Mar 15, 1967·Biochimica Et Biophysica Acta·S Su, P J Russell
Jan 1, 1980·Annual Review of Biochemistry·J R Knowles
Jan 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C N Falany
Mar 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C N Falany
Apr 17, 1998·Archives of Insect Biochemistry and Physiology·S M IsmailG Bhaskaran
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·T D SutherlandR Feyereisen
Dec 18, 1998·The Journal of Biological Chemistry·E VakianiJ Buck
Oct 16, 1999·Nature·J W Truman, L M Riddiford
Nov 24, 2001·Insect Biochemistry and Molecular Biology·A C Hinton, B D Hammock

❮ Previous
Next ❯

Citations

Jun 13, 2012·Biological & Pharmaceutical Bulletin·Masatoshi Iga, Hiroshi Kataoka
Aug 31, 2010·Journal of Pesticide Science·Shizuo G Kamita, Bruce D Hammock
Mar 8, 2005·Progress in Lipid Research·John W NewmanBruce D Hammock
Oct 6, 2005·Insect Biochemistry and Molecular Biology·Sheng LiDavid A Schooley
Mar 12, 2015·Acta Crystallographica. Section D, Biological Crystallography·P T ErskineJ B Cooper
Apr 13, 2017·Scientific Reports·Abudourusuli TusunGuirong Wang
Nov 30, 2020·International Journal of Biological Macromolecules·Haiyang XuPing Zhao
Feb 1, 2005·Insect Biochemistry and Molecular Biology·Qi-Rui ZhangSheng Li
Dec 7, 2021·Acta Crystallographica. Section F, Structural Biology Communications·Jingxu GuoJonathan B Cooper

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.